This title appears in the Scientific Report :
2003
Please use the identifier:
http://dx.doi.org/10.1002/cbic.200300631 in citations.
Selection of D-amino-acid peptides that bind to Alzheimer's disease amyloid peptide A beta(1-42) by mirror image phage display
Selection of D-amino-acid peptides that bind to Alzheimer's disease amyloid peptide A beta(1-42) by mirror image phage display
A mirror image phage display approach was used to identify novel and highly specific ligands for Alzheimer's disease amyloid peptide Abeta(1-42). A randomized 12-mer peptide library presented on M13 phages was screened for peptides with binding affinity for the mirror image of Abeta(1-42). Afte...
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Personal Name(s): | Wiesehan, K. |
---|---|
Buder, K. / Linke, R. P. / Patt, S. / Stoldt, M. / Unger, E. / Schmitt, B. / Bucci, E. / Willbold, D. | |
Contributing Institute: |
Biologische Strukturforschung; IBI-2 |
Published in: | ChemBioChem, 4 (2003) S. 748 - 753 |
Imprint: |
Weinheim
Wiley-VCH
2003
|
Physical Description: |
748 - 753 |
PubMed ID: |
12898626 |
DOI: |
10.1002/cbic.200300631 |
Document Type: |
Journal Article |
Research Program: |
Neurowissenschaften |
Series Title: |
ChemBioChem
4 |
Subject (ZB): | |
Publikationsportal JuSER |
A mirror image phage display approach was used to identify novel and highly specific ligands for Alzheimer's disease amyloid peptide Abeta(1-42). A randomized 12-mer peptide library presented on M13 phages was screened for peptides with binding affinity for the mirror image of Abeta(1-42). After four rounds of selection and amplification the peptides were enriched with a dominating consensus sequence. The mirror image of the most representative peptide (D-pep) was shown to bind Abeta(1-42) with a dissociation constant in the submicromolar range. Furthermore, in brain tissue sections derived from patients that suffered from Alzheimer's disease, amyloid plaques and leptomeningeal vessels containing Abeta amyloid were stained specifically with a fluorescence-labeled derivative of D-pep. Fibrillar deposits derived from other amyloidosis were not labeled by D-pep. Possible applications of this novel and highly specific Abeta ligand in diagnosis and therapy of Alzheimer's disease are discussed. |