This title appears in the Scientific Report :
2001
Please use the identifier:
http://dx.doi.org/10.1021/bp000164j in citations.
Please use the identifier: http://hdl.handle.net/2128/2436 in citations.
Human chymotrypsinogen B production with Pichia pastoris by integrated development of fermentation and downstream processing. 1: Fermation processing
Human chymotrypsinogen B production with Pichia pastoris by integrated development of fermentation and downstream processing. 1: Fermation processing
Based on an integrated approach of genetic engineering, fermentation process development, and downstream processing, a fermentative chymotrypsinogen B production process using recombinant Pichia pastoris is presented. Making use of the P. pastoris AOX1-promotor, the demand for methanol as the single...
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Personal Name(s): | Curvers, S. |
---|---|
Brixius, P. / Klauser, T. / Thömmes, J. / Weuster-Botz, D. / Takors, R. / Wandrey, C. | |
Contributing Institute: |
Biotechnologie 2; IBT-2 |
Published in: | Biotechnology progress, 17 (2001) S. 495 - 502 |
Imprint: |
Malden, MA
Wiley
2001
|
Physical Description: |
495 - 502 |
DOI: |
10.1021/bp000164j |
PubMed ID: |
11386871 |
Document Type: |
Journal Article |
Research Program: |
Verfahrenstechnik zur mikrobiellen Gewinnung von Primärmetaboliten |
Series Title: |
Biotechnology Progress
17 |
Subject (ZB): | |
Link: |
OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/2436 in citations.
Based on an integrated approach of genetic engineering, fermentation process development, and downstream processing, a fermentative chymotrypsinogen B production process using recombinant Pichia pastoris is presented. Making use of the P. pastoris AOX1-promotor, the demand for methanol as the single carbon source as well as an inducer of protein secretion enforced the use of an optimized feeding strategy by help of on-line analysis and an advanced controller algorithm. By using an experimental system of six parallel sparged column bioreactors, proteolytic product degradation could be minimized while also optimizing starting conditions for the following downstream processing. This optimization of process conditions resulted in the production of authentic chymotrypsinogen at a final concentration level of 480 mg.L(-)(1) in the whole broth and a biomass concentration of 150 g.L(-)(1) cell dry weight, thus comprising a space-time yield of 5.2 mg.L(-)(1).h(-)(1). Alternatively to the high cell density fermentation approach, a continuous fermentation process was developed to study the effects of reduced cell density toward oxygen demand, cooling energy, and biomass separation. This development led to a process with a highly increased space-time yield of 25 mg.L(-)(1).h(-)(1) while reducing the cell dry weight concentration from 150 g.L(-)(1) in fed-batch to 65 g.L(-)(1) in continuous cultivation. |