This title appears in the Scientific Report :
2008
Please use the identifier:
http://dx.doi.org/10.1209/0295-5075/83/46001 in citations.
Precise measurement of diffusion by multi-color dual-focus fluorescence correlation spectroscopy
Precise measurement of diffusion by multi-color dual-focus fluorescence correlation spectroscopy
Dual-focus. uorescence correlation spectroscopy is a method for precisely measuring the diffusion coefficient of fluorescing molecules close to the infinite dilution limit in a reference-free and absolute manner. We apply the method to determine the diffusion coefficients of three fluorescent dyes a...
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Personal Name(s): | Müller, C. B. |
---|---|
Loman, A. / Pacheco, V. / Koberling, F. / Willbold, D. / Richtering, W. / Enderlein, J. | |
Contributing Institute: |
Molekulare Biophysik; INB-2 Jülich-Aachen Research Alliance - Simulation Sciences; JARA-SIM |
Published in: | epl, 83 (2008) S. 46001 |
Imprint: |
Les Ulis
EDP Sciences
2008
|
Physical Description: |
46001 |
DOI: |
10.1209/0295-5075/83/46001 |
Document Type: |
Journal Article |
Research Program: |
Funktion und Dysfunktion des Nervensystems |
Series Title: |
Europhysics Letters
83 |
Subject (ZB): | |
Publikationsportal JuSER |
Dual-focus. uorescence correlation spectroscopy is a method for precisely measuring the diffusion coefficient of fluorescing molecules close to the infinite dilution limit in a reference-free and absolute manner. We apply the method to determine the diffusion coefficients of three fluorescent dyes across the visible spectrum. These values can be used as absolute reference standards for. uorescence correlation spectroscopy. In particular, it is found that the diffusion coefficient of the widely used reference dye Rhodamine 6G is by 37% larger than the value used in most publications on. uorescence correlation spectroscopy over the last three decades. Copyright (c) EPLA, 2008. |