This title appears in the Scientific Report :
2005
Please use the identifier:
http://dx.doi.org/10.1016/j.snb.2005.03.083 in citations.
Possibilities and limitations of label-free detection of DNA hybridization with field-effect-based devices
Possibilities and limitations of label-free detection of DNA hybridization with field-effect-based devices
A critical evaluation of the possibilities and limitations of the label-free detection of deoxyribonucleic acid (DNA) hybridization by means of field-effect-based devices is discussed. A new DNA-detection method is introduced, which utilizes an ion-sensitive field-effect device as transducer. The up...
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Personal Name(s): | Poghossian, A. |
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Cherstvy, A. / Ingebrandt, S. / Offenhäusser, A. / Schöning, M. J. | |
Contributing Institute: |
Institut für Bio- und Chemosensoren; ISG-2 Center of Nanoelectronic Systems for Information Technology; CNI Theorie II; IFF-TH-II |
Published in: |
Sensors and actuators |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2005
|
Physical Description: |
470 - 480 |
DOI: |
10.1016/j.snb.2005.03.083 |
Document Type: |
Journal Article |
Research Program: |
Kondensierte Materie Materialien, Prozesse und Bauelemente für die Mikro- und Nanoelektronik |
Series Title: |
Sensors and Actuators B
111-112 |
Subject (ZB): | |
Publikationsportal JuSER |
A critical evaluation of the possibilities and limitations of the label-free detection of deoxyribonucleic acid (DNA) hybridization by means of field-effect-based devices is discussed. A new DNA-detection method is introduced, which utilizes an ion-sensitive field-effect device as transducer. The upon the DNA hybridization induced redistribution of the ion concentration within the intermolecular spaces and/or the alteration of the ion sensitivity of the device is proposed as detection mechanism. The theoretical calculations predict a substantial change in the average ion concentration within the intermolecular spaces induced upon hybridization that are enough to obtain a detectable sensor signal. (c) 2005 Elsevier B.V. All rights reserved. |