This title appears in the Scientific Report :
2006
Please use the identifier:
http://dx.doi.org/10.1007/s00253-006-0562-8 in citations.
Determination of soluble and granular inorganic polyphosphate in Corynebacterium glutamicum
Determination of soluble and granular inorganic polyphosphate in Corynebacterium glutamicum
Corynebacterium glutamicum forms inorganic polyphosphate (poly P) that may occur as soluble (cytosolic) poly P and/or as volutin granules. A suitable method for monitoring soluble and granular poly P in C. glutamicum was developed and applied to C. glutamicum cells cultivated under different growth...
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Personal Name(s): | Klauth, P. |
---|---|
Pallerla, S. R. / Vidaurre, D. / Ralfs, C. / Wendisch, V. F. / Schoberth, S. M. | |
Contributing Institute: |
Agrosphäre; ICG-IV Biotechnologie 1; IBT-1 |
Published in: | Applied Microbiology and Biotechnology, 72 (2006) S. 1099 - 1106 |
Imprint: |
Berlin
Springer
2006
|
Physical Description: |
1099 - 1106 |
DOI: |
10.1007/s00253-006-0562-8 |
PubMed ID: |
16977467 |
Document Type: |
Journal Article |
Research Program: |
Biotechnologie Terrestrische Umwelt |
Series Title: |
Applied Microbiology and Biotechnology
72 |
Subject (ZB): | |
Publikationsportal JuSER |
Corynebacterium glutamicum forms inorganic polyphosphate (poly P) that may occur as soluble (cytosolic) poly P and/or as volutin granules. A suitable method for monitoring soluble and granular poly P in C. glutamicum was developed and applied to C. glutamicum cells cultivated under different growth conditions. Under phosphate-limiting conditions, C. glutamicum did not accumulate poly P, but it rebuilt its poly P storages when phosphate became available. The poly P content of C. glutamicum growing on glucose minimal medium with sufficient phosphate varied considerably during growth. While the poly P content was minimal in the midexponential growth phase, two maxima were observed in the early exponential growth phase and at entry into the stationary growth phase. Cells in the early exponential growth phase primarily contained granular poly P, while cells entering the stationary growth phase contained soluble, cytosolic poly P. These results and those obtained for C. glutamicum cells cultivated under hypo- or hyperosmotic conditions or during glutamate production revealed that the poly P content of C. glutamicum and the partitioning between cytosolic and granular forms of poly P are dynamics and depend on the growth conditions. |