This title appears in the Scientific Report :
2006
Please use the identifier:
http://dx.doi.org/10.1016/j.aca.2006.06.010 in citations.
Patterning chemical stimulation of reconstructed neuronal networks
Patterning chemical stimulation of reconstructed neuronal networks
A spatially resolved delivery of substances integrated with cell culture substrates shows promise for application in pharmacological assays, bioanalytical studies on cell signaling pathways and cell-based biosensors, where control over the extracellular biochemical environment with a cellular resolu...
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Personal Name(s): | Mourzina, Y. |
---|---|
Kaliaguine, D. / Schulte, P. / Offenhäusser, A. | |
Contributing Institute: |
Institut für Bio- und Chemosensoren; ISG-2 JARA-FIT; JARA-FIT Center of Nanoelectronic Systems for Information Technology; CNI |
Published in: | Analytica chimica acta, 575 (2006) S. 281 - 289 |
Imprint: |
Amsterdam
Elsevier Science
2006
|
Physical Description: |
281 - 289 |
PubMed ID: |
17723603 |
DOI: |
10.1016/j.aca.2006.06.010 |
Document Type: |
Journal Article |
Research Program: |
Grundlagen für zukünftige Informationstechnologien |
Series Title: |
Analytica Chimica Acta
575 |
Subject (ZB): | |
Publikationsportal JuSER |
A spatially resolved delivery of substances integrated with cell culture substrates shows promise for application in pharmacological assays, bioanalytical studies on cell signaling pathways and cell-based biosensors, where control over the extracellular biochemical environment with a cellular resolution is desirable. In this work, we studied a biohybrid system where rat embryonic cortical neuronal networks are reconstructed on microstructured silicon chips and interfaced to microfluidics. The design of cell-cell and cell-medium interactions in confined geometries is presented. We developed an aligned microcontact printing technique (AmicroCP) for poly(lysine)-extracellular matrix proteins on microstructured chips, which allows a high degree of geometrical control over the network architecture and alignment of the neuronal network with the microfluidic features of a substrate. Spatially resolved on-chip delivery of compounds with a cellular resolution is demonstrated by chemical stimulation of patterned rat cortical neurons within a network with a number of solutions of excitatory neurotransmitter glutamate delivered via microfluidics. The combination of the system described with a patch-clamp technique allowed both modulation of the biochemical environment on a cellular level and the monitoring of electrophysiological properties in the reconstructed rat embryonic cortical networks changed by this microenvironment. |