This title appears in the Scientific Report :
2007
Please use the identifier:
http://dx.doi.org/10.1529/biophysj.107.111328 in citations.
Cell Force Microscopy on Elastic Layers of Finite Thickness
Cell Force Microscopy on Elastic Layers of Finite Thickness
Forces applied by cells to substrates can be measured using soft substrates with embedded displacement markers. Traction forces are retrieved from microscopic images by determining the displacements of these markers and fitting the generating forces. Here we show that using elastic films of 5-10-mic...
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Personal Name(s): | Merkel, R. |
---|---|
Kirchgeßner, N. / Cesa, C. M. / Hoffmann, B. | |
Contributing Institute: |
Biomechanik; IBN-4 |
Published in: | Biophysical journal, 93 (2007) S. 3314 - 3323 |
Imprint: |
New York, NY
Rockefeller Univ. Press
2007
|
Physical Description: |
3314 - 3323 |
PubMed ID: |
17660320 |
DOI: |
10.1529/biophysj.107.111328 |
Document Type: |
Journal Article |
Research Program: |
Kondensierte Materie |
Series Title: |
Biophysical Journal
93 |
Subject (ZB): | |
Publikationsportal JuSER |
Forces applied by cells to substrates can be measured using soft substrates with embedded displacement markers. Traction forces are retrieved from microscopic images by determining the displacements of these markers and fitting the generating forces. Here we show that using elastic films of 5-10-microm thickness one can improve the spatial resolution of the technique. To this end we derived explicit equations for the mechanical response of an elastic layer of finite thickness to point forces. Moreover, these equations allow highly accurate force measurements on eukaryotic cells on films where finite thickness effects are relevant (below approximately 60 microm). |