This title appears in the Scientific Report :
2007
Please use the identifier:
http://dx.doi.org/10.1111/j.1365-2958.2007.05884.x in citations.
Identification of an alpha (1 -> 6) mannopyranosyltransferase (MptA), involved in Corynebacterium glutamicum lipomanann biosynthesis, and identification of its orthologue in Mycobacterium tuberculosis
Identification of an alpha (1 -> 6) mannopyranosyltransferase (MptA), involved in Corynebacterium glutamicum lipomanann biosynthesis, and identification of its orthologue in Mycobacterium tuberculosis
Corynebacterium glutamicum and Mycobacterium tuberculosis share a similar cell wall architecture, and the availability of their genome sequences has enabled the utilization of C. glutamicum as a model for the identification and study of, otherwise essential, mycobacterial genes involved in lipomanna...
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Personal Name(s): | Mishra, A. K. |
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Alderwick, L. J. / Rittmann, D. / Tatituri, R. V. V. / Nigou, J. / Gilleron, M. / Eggeling, L. / Besra, G. S. | |
Contributing Institute: |
Biotechnologie 1; IBT-1 |
Published in: | Molecular microbiology, 65 (2007) S. 1503 - 1517 |
Imprint: |
Oxford [u.a.]
Wiley-Blackwell
2007
|
Physical Description: |
1503 - 1517 |
PubMed ID: |
17714444 |
DOI: |
10.1111/j.1365-2958.2007.05884.x |
Document Type: |
Journal Article |
Research Program: |
Biotechnologie |
Series Title: |
Molecular Microbiology
65 |
Subject (ZB): | |
Publikationsportal JuSER |
Corynebacterium glutamicum and Mycobacterium tuberculosis share a similar cell wall architecture, and the availability of their genome sequences has enabled the utilization of C. glutamicum as a model for the identification and study of, otherwise essential, mycobacterial genes involved in lipomannan (LM) and lipoarabinomannan (LAM) biosynthesis. We selected the putative glycosyltransferase-Rv2174 from M. tuberculosis and deleted its orthologue NCgl2093 from C. glutamicum. This resulted in the formation of a novel truncated lipomannan (Cg-t-LM) and a complete ablation of LM/LAM biosynthesis. Purification and characterization of Cg-t-LM revealed an overall decrease in molecular mass, a reduction of alpha(1-->6) and alpha(1-->2) glycosidic linkages illustrating a reduced degree of branching compared with wild-type LM. The deletion mutant's biochemical phenotype was fully complemented by either NCgl2093 or Rv2174. Furthermore, the use of a synthetic neoglycolipid acceptor in an in vitro cell-free assay utilizing the sugar donor beta-D-mannopyranosyl-1-monophosphoryl-decaprenol together with the neoglycolipid acceptor alpha-D-Manp-(1-->6)-alpha-D-Manp-O-C8 as a substrate, confirmed NCgl2093 and Rv2174 as an alpha(1-->6) mannopyranosyltransferase (MptA), involved in the latter stages of the biosynthesis of the alpha(1-->6) mannan core of LM. Altogether, these studies have identified a new mannosyltransferase, MptA, and they shed further light on the biosynthesis of LM/LAM in Corynebacterianeae. |