This title appears in the Scientific Report :
2008
Please use the identifier:
http://dx.doi.org/10.1007/s00216-007-1647-7 in citations.
Miniaturized planar lipid bilayer: increased stability, low electric noise and fast fluid perfusion
Miniaturized planar lipid bilayer: increased stability, low electric noise and fast fluid perfusion
A microfluidic device was designed allowing the formation of a planar lipid bilayer across a micron-sized aperture in a glass slide sandwiched between two polydimethylsiloxane channel systems. By flushing giant unilamellar vesicles through a 500-microm-wide channel above the hole, we were able to fo...
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Personal Name(s): | Mach, T. |
---|---|
Chimerel, C. / Fritz, J. / Fertig, N. / Winterhalter, M. / Fütterer, C. | |
Contributing Institute: |
Zelluläre Biophysik; INB-1 |
Published in: | Analytical and bioanalytical chemistry, 390 (2008) S. 841 - 846 |
Imprint: |
Berlin
Springer
2008
|
Physical Description: |
841 - 846 |
PubMed ID: |
17972068 |
DOI: |
10.1007/s00216-007-1647-7 |
Document Type: |
Journal Article |
Research Program: |
Funktion und Dysfunktion des Nervensystems |
Series Title: |
Analytical and Bioanalytical Chemistry
390 |
Subject (ZB): | |
Publikationsportal JuSER |
A microfluidic device was designed allowing the formation of a planar lipid bilayer across a micron-sized aperture in a glass slide sandwiched between two polydimethylsiloxane channel systems. By flushing giant unilamellar vesicles through a 500-microm-wide channel above the hole, we were able to form a planar lipid bilayer across the hole, resulting in a giga-seal. We demonstrate incorporation of biological nanopores into the bilayer. This miniaturized system offers noise recordings comparable to open head-stage noise (under 1 pA RMS at 10 kHz), fast precision perfusion on each side of the membrane and the use of nanoliter analyte volumes. This technique shows a promising potential for automation and parallelization of electrophysiological setups. |