This title appears in the Scientific Report :
2008
Please use the identifier:
http://dx.doi.org/10.1039/b803554g in citations.
LA-ICP-MS studies of zinc exchange by copper in bovine serum albumin using an isotopic enriched copper tracer
LA-ICP-MS studies of zinc exchange by copper in bovine serum albumin using an isotopic enriched copper tracer
In the present work the binding of Cu and Zn on bovine serum proteins was studied using a tracer experiment. One dimensional BN- PAGE gels with separated bovine serum proteins after gel electrophoresis were doped with an enriched isotope copper tracer (65 Cu) as a function of time (from 30 s up to 2...
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Personal Name(s): | Becker, J. Su. |
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Pozebon, D. / Dressler, V. L. / Lobinski, R. / Becker, J. Sa. | |
Contributing Institute: |
Zentralabteilung für Chemische Analysen; ZCH |
Published in: | Journal of analytical atomic spectrometry, 23 (2008) S. 1076 - 1082 |
Imprint: |
Cambridge
ChemSoc
2008
|
Physical Description: |
1076 - 1082 |
DOI: |
10.1039/b803554g |
Document Type: |
Journal Article |
Research Program: |
Funktion und Dysfunktion des Nervensystems |
Series Title: |
Journal of Analytical Atomic Spectrometry
23 |
Subject (ZB): | |
Publikationsportal JuSER |
In the present work the binding of Cu and Zn on bovine serum proteins was studied using a tracer experiment. One dimensional BN- PAGE gels with separated bovine serum proteins after gel electrophoresis were doped with an enriched isotope copper tracer (65 Cu) as a function of time (from 30 s up to 24 h). In several protein bands in 1D gel before tracer experiment metal ions (mostly Zn) were found by LA-ICP-MS. After experiments using enriched 65 Cu tracer 65 Cu/ 63 Cu isotope ratios with changed isotope ratio were measured by LA- ICP- MS. The isotope ratio measurements of copper by LA- ICP- MS as a function of time indicate a fast exchange of Zn bonded on bovine serum albumin. This experimental finding demonstrates the formation of copper- binding proteins during the tracer experiments in the 1D gel. It was attempted to identify the protein bands from bovine serum proteins separated by 1D gel electrophoresis by using biopolymer mass spectrometry with MALDI-TOF-MS after excision from the 1D gel and tryptic digestion. |