This title appears in the Scientific Report :
2016
Please use the identifier:
http://dx.doi.org/10.1002/elsc.201500142 in citations.
Plug flow versus stirred tank reactor flow characteristics in two-compartment scale-down bioreactor: Setup-specific influence on the metabolic phenotype and bioprocess performance of Corynebacterium glutamicum
Plug flow versus stirred tank reactor flow characteristics in two-compartment scale-down bioreactor: Setup-specific influence on the metabolic phenotype and bioprocess performance of Corynebacterium glutamicum
In the last two decades, scale-down studies based on compartmented reactor setups became the standard procedure to mimic inhomogeneous cultivation conditions. In the academic field and with application to industrial-scale, two basic scale-down bioreactor configurations both showing a stirred tank re...
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Personal Name(s): | Limberg, Michael |
---|---|
Pooth, Viola / Wiechert, Wolfgang / Oldiges, Marco (Corresponding author) | |
Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | Engineering in life sciences, 16 (2016) 7, S. 610 - 619 |
Imprint: |
Weinheim
Wiley-VCH
2016
|
DOI: |
10.1002/elsc.201500142 |
Document Type: |
Journal Article |
Research Program: |
Biotechnology |
Publikationsportal JuSER |
In the last two decades, scale-down studies based on compartmented reactor setups became the standard procedure to mimic inhomogeneous cultivation conditions. In the academic field and with application to industrial-scale, two basic scale-down bioreactor configurations both showing a stirred tank reactor (STR) as main compartment predominate this research field. The connection to a plug flow reactor (PFR) generates oscillatory gradients with a distinct residence time of the culture, while the STR provides a broad residence time distribution leading to more heterogeneous oscillations. The influence of these opposed hydrodynamic profiles for their applicability for scale-down bioreactor setups as well as their specific influence on the metabolic phenotype of l-lysine producing Corynebacterium glutamicum DM1800 strain was investigated. Batch cultivations under oscillatory oxygen deprivation and substrate excess were carried out in STR–PFR and STR–STR scale-down devices. In both setups, the induced inhomogeneity resulted in a reduction of growth rate and increased the l-lactate and l-glutamate by-product formation, while biomass and product yields stayed nearly constant. Apart from differing side-product levels, very similar results were observed when comparing the metabolic phenotype and bioprocess performance of STR–PFR and STR–STR configuration, although opposed back mixing profiles were present. |