This title appears in the Scientific Report :
2016
Please use the identifier:
http://dx.doi.org/10.1080/15592324.2015.1119962 in citations.
Benzoxazolinone detoxification by N-Glucosylation: The multi-compartment-network of Zea mays L.
Benzoxazolinone detoxification by N-Glucosylation: The multi-compartment-network of Zea mays L.
The major detoxification product in maize roots after 24 h benzoxazolin-2(3H)-one (BOA) exposure was identified as glucoside carbamate resulting from rearrangement of BOA-N-glucoside, but the pathway of N-glucosylation, enzymes involved and the site of synthesis were previously unknown. Assaying who...
| Personal Name(s): | Schulz, Margot (Corresponding author) |
|---|---|
| Filary, Barbara / Kühn, Sabine / Colby, Thomas / Harzen, Anne / Schmidt, Jürgen / Sicker, Dieter / Hennig, Lothar / Hofmann, Diana / Disko, Ulrich / Anders, Nico | |
| Contributing Institute: |
Agrosphäre; IBG-3 |
| Published in: | Plant signaling & behavior, 11 (2016) 1, S. e1119962 |
| Imprint: |
Austin, Tex.
Landes Bioscience
2016
|
| DOI: |
10.1080/15592324.2015.1119962 |
| PubMed ID: |
26645909 |
| Document Type: |
Journal Article |
| Research Program: |
Terrestrial Systems: From Observation to Prediction |
| Publikationsportal JuSER |
|
The major detoxification product in maize roots after 24 h benzoxazolin-2(3H)-one (BOA) exposure was identified as glucoside carbamate resulting from rearrangement of BOA-N-glucoside, but the pathway of N-glucosylation, enzymes involved and the site of synthesis were previously unknown. Assaying whole cell proteins revealed the necessity of H2O2 and Fe2+ ions for glucoside carbamate production. Peroxidase produced BOA radicals are apparently formed within the extraplastic space of the young maize root. Radicals seem to be the preferred substrate for N-glucosylation, either by direct reaction with glucose or, more likely, the N-glucoside is released by glucanase/glucosidase catalyzed hydrolysis from cell wall components harboring fixed BOA. [...] |