This title appears in the Scientific Report :
2016
Please use the identifier:
http://dx.doi.org/10.1021/acs.langmuir.5b04644 in citations.
Reconstitution of Fusion Proteins in Supported Lipid Bilayers for the Study of Cell Surface Receptor–Ligand Interactions in Cell–Cell Contact
Reconstitution of Fusion Proteins in Supported Lipid Bilayers for the Study of Cell Surface Receptor–Ligand Interactions in Cell–Cell Contact
Bioactive molecules such as adhesion ligands, growth factors, or enzymes play an important role in modulating cell behavior such as cell adhesion, spreading, and differentiation. Deciphering the mechanism of ligand-mediated cell adhesion and associated signaling is of great interest not only for fun...
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Personal Name(s): | Ghosh Moulick, R. |
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Afanasenkau, D. / Choi, S.-E. / Albers, J. / Lange, W. / Maybeck, V. / Utesch, T. / Offenhäusser, Andreas (Corresponding author) | |
Contributing Institute: |
Bioelektronik; ICS-8 |
Published in: | Langmuir, 32 (2016) 14, S. 3462 - 3469 |
Imprint: |
Washington, DC
ACS Publ.
2016
|
PubMed ID: |
26986674 |
DOI: |
10.1021/acs.langmuir.5b04644 |
Document Type: |
Journal Article |
Research Program: |
GRK 1572: Bionik - Interaktionen über Grenzflächen zur Außenwelt Engineering Cell Function |
Publikationsportal JuSER |
Bioactive molecules such as adhesion ligands, growth factors, or enzymes play an important role in modulating cell behavior such as cell adhesion, spreading, and differentiation. Deciphering the mechanism of ligand-mediated cell adhesion and associated signaling is of great interest not only for fundamental biophysical investigations but also for applications in medicine and biotechnology. In the presented work, we developed a new biomimetic platform that enables culturing primary neurons and testing cell surface–receptor ligand interactions in cell–cell contacts as, e.g., in neuronal synapses. This platform consists of a supported lipid bilayer modified with incorporated neuronal adhesion proteins conjugated with the Fc-domain of IgG (ephrin A5 Fc-chimera). We extensively characterized properties of these protein containing bilayers using fluorescence recovery after photobleaching (FRAP), quartz crystal microbalance with dissipation (QCM-D), and immunostaining. We conclude that the Fc-domain is the part responsible for the incorporation of the protein into the bilayer. The biomimetic platform prepared by this new approach was able to promote neuronal cell adhesion and maintain growth as well as facilitate neuronal maturation as shown by electrophysiological measurements. We believe that our approach can be extended to insert other proteins to create a general culture platform for neurons and other cell types. |