This title appears in the Scientific Report :
2017
Please use the identifier:
http://dx.doi.org/10.1016/j.micron.2016.11.002 in citations.
Please use the identifier: http://hdl.handle.net/2128/15763 in citations.
How to image cell adhesion on soft polymers?
How to image cell adhesion on soft polymers?
Here, we present a method to investigate cell adhesion on soft, non-conducting polymers that are implant candidate materials. Neuronal cells were grown on two elastomers (polydimethylsiloxane (PDMS) and Ecoflex®) and prepared for electron microscopy. The samples were treated with osmium tetroxide (O...
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Personal Name(s): | Seyock, Silke (First author) |
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Maybeck, Vanessa / Offenhäusser, Andreas (Corresponding author) | |
Contributing Institute: |
Bioelektronik; PGI-8 Bioelektronik; ICS-8 |
Published in: | Micron, 92 (2017) S. 39-42 |
Imprint: |
New York, NY [u.a.]
Elsevier
2017
|
DOI: |
10.1016/j.micron.2016.11.002 |
PubMed ID: |
27866099 |
Document Type: |
Journal Article |
Research Program: |
Engineering Cell Function |
Link: |
Restricted Restricted OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/15763 in citations.
Here, we present a method to investigate cell adhesion on soft, non-conducting polymers that are implant candidate materials. Neuronal cells were grown on two elastomers (polydimethylsiloxane (PDMS) and Ecoflex®) and prepared for electron microscopy. The samples were treated with osmium tetroxide (OsO4) and uranylacetate (UrAc). Best results can be achieved when the polymers were coated with a thin iridium layer before the cell culture. This was done to emphasize the usage of soft polymers as supports for implant electrodes. A good contrast and the adhesion of the cells on soft polymers could be visualized. |