This title appears in the Scientific Report :
2017
Please use the identifier:
http://dx.doi.org/10.1016/j.clinbiochem.2016.11.001 in citations.
sFIDA automation yields sub-femtomolar limit of detection for Aβ aggregates in body fluids
sFIDA automation yields sub-femtomolar limit of detection for Aβ aggregates in body fluids
Objectives: Alzheimer's disease (AD) is a neurodegenerative disorder with yet non-existent therapeutic and limited diagnostic options. Reliable biomarker-based AD diagnostics are of utmost importance for the development and application of therapeutic substances. Wehave previously introduced a p...
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Personal Name(s): | Herrmann, Yvonne |
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Kulawik, Andreas / Kühbach, Katja / Hülsemann, Maren / Peters, Luriano / Bujnicki, Tuyen / Kravchenko, Kateryna / Linnartz, Christina / Willbold, Johannes / Zafiu, Christian / Bannach, Oliver / Willbold, Dieter (Corresponding author) | |
Contributing Institute: |
Strukturbiochemie; ICS-6 |
Published in: | Clinical biochemistry, 50 (2017) 4-5, S. 244-247 |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2017
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PubMed ID: |
27823959 |
DOI: |
10.1016/j.clinbiochem.2016.11.001 |
Document Type: |
Journal Article |
Research Program: |
Physical Basis of Diseases |
Publikationsportal JuSER |
Objectives: Alzheimer's disease (AD) is a neurodegenerative disorder with yet non-existent therapeutic and limited diagnostic options. Reliable biomarker-based AD diagnostics are of utmost importance for the development and application of therapeutic substances. Wehave previously introduced a platformtechnology designated ‘sFIDA’ for the quantitation of amyloid β peptide (Aβ) aggregates as AD biomarker. In this study we implemented the sFIDA assay on an automated platform to enhance robustness and performance of the assay.Design and methods: In sFIDA (surface-based fluorescence intensity distribution analysis) Aβ species are immobilized by a capture antibody to a glass surface. Aβ aggregates are then multiply loaded with fluorescent antibodies and quantitated by high resolution fluorescence microscopy. As a model system for Aβ aggregates, weused Aβ-conjugated silica nanoparticles (Aβ-SiNaPs) diluted in PBS buffer and cerebrospinal fluid, respectively. Automation of the assay was realized on a liquid handling system in combination with a microplate washer.Results: The automation of the sFIDA assay results in improved intra-assay precision, linearity and sensitivity in comparison to the manual application, and achieved a limit of detection in the sub-femtomolar range.Conclusions: Automation improves the precision and sensitivity of the sFIDA assay, which is a prerequisite for high-throughput measurements and future application of the technology in routine AD diagnostics. |