This title appears in the Scientific Report :
2016
Please use the identifier:
http://dx.doi.org/10.1093/chemse/bjw077 in citations.
Elevated Cytosolic Cl − Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons
Elevated Cytosolic Cl − Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons
In rodents, the vomeronasal system controls social and sexual behavior. However, several mechanistic aspects of sensory signaling in the vomeronasal organ remain unclear. Here, we investigate the biophysical basis of a recently proposed vomeronasal signal transduction component—a Ca 2+ -activated Cl...
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Personal Name(s): | Untiet, Verena |
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Moeller, Lisa M. / Ibarra-Soria, Ximena / Sánchez-Andrade, Gabriela / Stricker, Miriam / Neuhaus, Eva M. / Logan, Darren W. / Gensch, Thomas / Spehr, Marc (Corresponding author) | |
Contributing Institute: |
Zelluläre Biophysik; ICS-4 |
Published in: | Chemical senses, 41 (2016) 8, S. 669 - 676 |
Imprint: |
Oxford
Oxford Univ. Press
2016
|
DOI: |
10.1093/chemse/bjw077 |
PubMed ID: |
27377750 |
Document Type: |
Journal Article |
Research Program: |
Physical Basis of Diseases |
Publikationsportal JuSER |
In rodents, the vomeronasal system controls social and sexual behavior. However, several mechanistic aspects of sensory signaling in the vomeronasal organ remain unclear. Here, we investigate the biophysical basis of a recently proposed vomeronasal signal transduction component—a Ca 2+ -activated Cl − current. As the physiological role of such a current is a direct function of the Cl − equilibrium potential, we determined the intracellular Cl − concentration in dendritic knobs of vomeronasal neurons. Quantitative fluorescence lifetime imaging of a Cl − -sensitive dye at the apical surface of the intact vomeronasal neuroepithelium revealed increased cytosolic Cl − levels in dendritic knobs, a substantially lower Cl − concentration in vomeronasal sustentacular cells, and an apparent Cl − gradient in vomeronasal neurons along their dendritic apicobasal axis. Together, our data provide a biophysical basis for sensory signal amplification in vomeronasal neuron microvilli by opening Ca 2+ -activated Cl − channels. |