This title appears in the Scientific Report :
2017
Please use the identifier:
http://hdl.handle.net/2128/13767 in citations.
Please use the identifier: http://dx.doi.org/10.1186/s13068-017-0711-6 in citations.
A framework for accelerated phototrophic bioprocess development: integration of parallelized microscale cultivation, laboratory automation and Kriging-assisted experimental design
A framework for accelerated phototrophic bioprocess development: integration of parallelized microscale cultivation, laboratory automation and Kriging-assisted experimental design
BackgroundEven though microalgae-derived biodiesel has regained interest within the last decade, industrial production is still challenging for economic reasons. Besides reactor design, as well as value chain and strain engineering, laborious and slow early-stage parameter optimization represents a...
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Personal Name(s): | Morschett, Holger |
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Freier, Lars / Rohde, Jannis / Wiechert, Wolfgang / von Lieres, Eric / Oldiges, Marco (Corresponding author) | |
Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | Biotechnology for biofuels, 10 (2017) 1, S. 26 |
Imprint: |
London
BioMed Central
2017
|
PubMed ID: |
28163783 |
DOI: |
10.1186/s13068-017-0711-6 |
Document Type: |
Journal Article |
Research Program: |
Innovative Synergisms |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1186/s13068-017-0711-6 in citations.
BackgroundEven though microalgae-derived biodiesel has regained interest within the last decade, industrial production is still challenging for economic reasons. Besides reactor design, as well as value chain and strain engineering, laborious and slow early-stage parameter optimization represents a major drawback.ResultsThe present study introduces a framework for the accelerated development of phototrophic bioprocesses. A state-of-the-art micro-photobioreactor supported by a liquid-handling robot for automated medium preparation and product quantification was used. To take full advantage of the technology’s experimental capacity, Kriging-assisted experimental design was integrated to enable highly efficient execution of screening applications. The resulting platform was used for medium optimization of a lipid production process using Chlorella vulgaris toward maximum volumetric productivity. Within only four experimental rounds, lipid production was increased approximately threefold to 212 ± 11 mg L−1 d−1. Besides nitrogen availability as a key parameter, magnesium, calcium and various trace elements were shown to be of crucial importance. Here, synergistic multi-parameter interactions as revealed by the experimental design introduced significant further optimization potential.ConclusionsThe integration of parallelized microscale cultivation, laboratory automation and Kriging-assisted experimental design proved to be a fruitful tool for the accelerated development of phototrophic bioprocesses. By means of the proposed technology, the targeted optimization task was conducted in a very timely and material-efficient manner. |