This title appears in the Scientific Report :
2017
Please use the identifier:
http://hdl.handle.net/2128/14606 in citations.
Please use the identifier: http://dx.doi.org/10.3390/molecules22040655 in citations.
Protein Stability and Unfolding Following Glycine Radical Formation
Protein Stability and Unfolding Following Glycine Radical Formation
Glycine (Gly) residues are particularly susceptible to hydrogen abstraction; which results in the formation of the capto-dative stabilized Cα-centered Gly radical (GLR) on the protein backbone. We examined the effect of GLR formation on the structure of the Trp cage; tryptophan zipper; and the villi...
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Personal Name(s): | Owen, Michael (Corresponding author) |
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Csizmadia, Imre G. / Viskolcz, Béla / Strodel, Birgit | |
Contributing Institute: |
Strukturbiochemie; ICS-6 |
Published in: | Molecules, 22 (2017) 4, S. 655 |
Imprint: |
Basel
MDPI
2017
|
PubMed ID: |
28422069 |
DOI: |
10.3390/molecules22040655 |
Document Type: |
Journal Article |
Research Program: |
Physical Basis of Diseases |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.3390/molecules22040655 in citations.
Glycine (Gly) residues are particularly susceptible to hydrogen abstraction; which results in the formation of the capto-dative stabilized Cα-centered Gly radical (GLR) on the protein backbone. We examined the effect of GLR formation on the structure of the Trp cage; tryptophan zipper; and the villin headpiece; three fast-folding and stable miniproteins; using all-atom (OPLS-AA) molecular dynamics simulations. Radicalization changes the conformation of the GLR residue and affects both neighboring residues but did not affect the stability of the Trp zipper. The stability of helices away from the radical center in villin were also affected by radicalization; and GLR in place of Gly15 caused the Trp cage to unfold within 1 µs. These results provide new evidence on the destabilizing effects of protein oxidation by reactive oxygen species. |