This title appears in the Scientific Report :
2017
Please use the identifier:
http://hdl.handle.net/2128/14552 in citations.
Please use the identifier: http://dx.doi.org/10.1021/acs.cgd.6b01631 in citations.
Integral Membrane Proteins Can Be Crystallized Directly from Nanodiscs
Integral Membrane Proteins Can Be Crystallized Directly from Nanodiscs
Membrane-like nanodiscs (ND) have become an important tool for the cell-free expression, solubilization, folding, and in vitro structural and functional studies of membrane proteins (MPs). Direct crystallization of MPs embedded in NDs would be of high importance for structural biology. However, desp...
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Personal Name(s): | Nikolaev, Michael |
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Round, E. / Gushchin, Ivan / Polovinkin, V. / Balandin, Taras / Kuzmichev / Shevchenko, Vitaly / Borshchevskiy, Valentin / Kuklin, A. / Round, A. / Bernhard, F. / Willbold, Dieter / Büldt, Georg / Gordeliy, Valentin (Corresponding author) | |
Contributing Institute: |
Strukturbiochemie; ICS-6 |
Published in: | Journal of crystal growth, 17 (2017) 3, S. 945–948 |
Imprint: |
Amsterdam [u.a.]
Elsevier
2017
|
DOI: |
10.1021/acs.cgd.6b01631 |
Document Type: |
Journal Article |
Research Program: |
Functional Macromolecules and Complexes |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1021/acs.cgd.6b01631 in citations.
Membrane-like nanodiscs (ND) have become an important tool for the cell-free expression, solubilization, folding, and in vitro structural and functional studies of membrane proteins (MPs). Direct crystallization of MPs embedded in NDs would be of high importance for structural biology. However, despite considerable efforts we have been as yet unable to obtain crystals suitable for X-ray crystallography. In the present work, we show that an ND-trapped MP can be transferred into the cubic phase and crystallized in meso. Bacteriorhodopsin (BR) reconstituted into nanodiscs was mixed with a lipidic mesophase and crystallization was induced by adding a precipitant. The resulting crystals diffract beyond 1.8 Å. The structure of BR was solved at 1.9 Å and found to be indistinguishable from previous structures obtained with the protein solubilized in detergent. We suggest the proposed protocol of in meso crystallization to be generally applicable to ND-trapped MPs. |