This title appears in the Scientific Report :
2017
Tissue specific ploidy variation in sexual and apomictic seeds
Tissue specific ploidy variation in sexual and apomictic seeds
Polyploidy effects are often examined at the level of a whole organism or across species, however ploidy variation occurs also at intra-individual level and can be limited to a specific tissue, e.g. endosperm tissue in the seeds of flowering plants. Sexual reproduction typically involves double fert...
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Personal Name(s): | Paczesniak, D. (Corresponding author) |
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Pellino, M. / Lovell, J. T. / Guenter, D. / Jahnke, Siegfried / Roussel, J. / Fischbach, Andreas | |
Contributing Institute: |
Pflanzenwissenschaften; IBG-2 |
Imprint: |
2017
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Conference: | Annual Meeting of the Society for Molecular Biology and Evolution, Austin, Texas (USA), 2017-07-02 - 2017-07-06 |
Document Type: |
Poster |
Research Program: |
Deutsches Pflanzen Phänotypisierungsnetzwerk Plant Science |
Publikationsportal JuSER |
Polyploidy effects are often examined at the level of a whole organism or across species, however ploidy variation occurs also at intra-individual level and can be limited to a specific tissue, e.g. endosperm tissue in the seeds of flowering plants. Sexual reproduction typically involves double fertilization, whereby the egg and central cells of the ovary are fertilized to produce a diploid embryo and a triploid endosperm (nutritious tissue with 2 maternal and 1 paternal genomes). In the genus Boechera, a wild relative of Arabidopsis, both sexual and apomictic (i.e. reproducing asexually via seed) lineages are found. Diploid apomictic lineages produce a meiotically-unreduced diploid egg cell which develops parthenogenetically (without fertilization) into an embryo that is genetically identical to the mother plant, whereas endosperm is pseudogamous (i.e. requires fertilization by diploid pollen) and typically hexaploid, with 2:1 maternal to paternal genome ratio. Hence the seeds of sexual and apomictic Boechera have the same embryo ploidy (2x), but differ with respect to endosperm ploidy (3x and 6x).We are interested in understanding effects of ploidy variation on endosperm function and plant fitness, in the context of sexual and apomictic reproduction. To do so we have (1) compared gene expression patterns between developing hexaploid (apomictic) and triploid (sexual) endosperm in the seeds from multiple apomictic and sexual genotypes using a custom Boechera microarray and live microdissected endosperm tissue, and (2) have examined the effects of genotype, ploidy and parent of origin effects as potential factors underlying phenotypic variation in seed size, an agriculturally important trait. |