This title appears in the Scientific Report :
2017
Please use the identifier:
http://hdl.handle.net/2128/15987 in citations.
Please use the identifier: http://dx.doi.org/10.3762/bjoc.13.148 in citations.
2-Methyl-2,4-pentanediol (MPD) boosts as detergent-substitute the performance of ß-barrel hybrid catalyst for phenylacetylene polymerization
2-Methyl-2,4-pentanediol (MPD) boosts as detergent-substitute the performance of ß-barrel hybrid catalyst for phenylacetylene polymerization
Covering hydrophobic regions with stabilization agents to solubilize purified transmembrane proteins is crucial for their application in aqueous media. The small molecule 2-methyl-2,4-pentanediol (MPD) was used to stabilize the transmembrane protein Ferric hydroxamate uptake protein component A (Fhu...
Saved in:
Personal Name(s): | Kinzel, Julia |
---|---|
Sauer, Daniel F / Bocola, Marco / Arlt, Marcus / Mirzaei Garakani, Tayebeh / Thiel, Andreas / Beckerle, Klaus / Polen, Tino / Okuda, Jun / Schwaneberg, Ulrich (Corresponding author) | |
Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | Beilstein journal of organic chemistry, 13 (2017) S. 1498 - 1506 |
Imprint: |
Frankfurt, Main
Beilstein-Institut zur Förderung der Chemischen Wissenschaften
2017
|
DOI: |
10.3762/bjoc.13.148 |
PubMed ID: |
28845193 |
Document Type: |
Journal Article |
Research Program: |
Biotechnology |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.3762/bjoc.13.148 in citations.
Covering hydrophobic regions with stabilization agents to solubilize purified transmembrane proteins is crucial for their application in aqueous media. The small molecule 2-methyl-2,4-pentanediol (MPD) was used to stabilize the transmembrane protein Ferric hydroxamate uptake protein component A (FhuA) utilized as host for the construction of a rhodium-based biohybrid catalyst. Unlike commonly used detergents such as sodium dodecyl sulfate or polyethylene polyethyleneglycol, MPD does not form micelles in solution. Molecular dynamics simulations revealed the effect and position of stabilizing MPD molecules. The advantage of the amphiphilic MPD over micelle-forming detergents is demonstrated in the polymerization of phenylacetylene, showing a ten-fold increase in yield and increased molecular weights. |