This title appears in the Scientific Report :
2018
Please use the identifier:
http://hdl.handle.net/2128/27263 in citations.
Please use the identifier: http://dx.doi.org/10.1093/cercor/bhx352 in citations.
Morphological and Functional Characterization of Non-fast-Spiking GABAergic Interneurons in Layer 4 Microcircuitry of Rat Barrel Cortex
Morphological and Functional Characterization of Non-fast-Spiking GABAergic Interneurons in Layer 4 Microcircuitry of Rat Barrel Cortex
GABAergic interneurons are notorious for their heterogeneity, despite constituting a small fraction of the neuronal population in the neocortex. Classification of interneurons is crucial for understanding their widespread cortical functions as they provide a complex and dynamic network, balancing ex...
Saved in:
Personal Name(s): | Emmenegger, Vishalini |
---|---|
Qi, Guanxiao / Wang, Haijun / Feldmeyer, Dirk (Corresponding author) | |
Contributing Institute: |
Jara-Institut Brain structure-function relationships; INM-10 |
Published in: | Cerebral cortex, 28 (2018) 4, S. 1439 - 1457 |
Imprint: |
Oxford
Oxford Univ. Press
2018
|
DOI: |
10.1093/cercor/bhx352 |
Document Type: |
Journal Article |
Research Program: |
ohne Topic |
Link: |
Get full text Get full text Get full text Get full text OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1093/cercor/bhx352 in citations.
GABAergic interneurons are notorious for their heterogeneity, despite constituting a small fraction of the neuronal population in the neocortex. Classification of interneurons is crucial for understanding their widespread cortical functions as they provide a complex and dynamic network, balancing excitation and inhibition. Here, we investigated different types of non-fast-spiking (nFS) interneurons in Layer 4 (L4) of rat barrel cortex using whole-cell patch-clamp recordings with biocytin-filling. Based on a quantitative analysis on a combination of morphological and electrophysiological parameters, we identified 5 distinct types of L4 nFS interneurons: 1) trans-columnar projecting interneurons, 2) locally projecting non-Martinotti-like interneurons, 3) supra-granular projecting Martinotti-like interneurons, 4) intra-columnar projecting VIP-like interneurons, and 5) locally projecting neurogliaform-like interneurons. Trans-columnar projecting interneurons are one of the most striking interneuron types, which have not been described so far in Layer 4. They feature extensive axonal collateralization not only in their home barrel but also in adjacent barrels. Furthermore, we identified that most of the L4 nFS interneurons express somatostatin, while few are positive for the transcription factor Prox1. The morphological and electrophysiological characterization of different L4 nFS interneuron types presented here provides insights into their synaptic connectivity and functional role in cortical information processing. |