This title appears in the Scientific Report :
2018
Please use the identifier:
http://dx.doi.org/10.1111/nph.15265 in citations.
Please use the identifier: http://hdl.handle.net/2128/19656 in citations.
Dual function of a secreted fungalysin metalloprotease in Ustilago maydis
Dual function of a secreted fungalysin metalloprotease in Ustilago maydis
Fungalysins from several phytopathogenic fungi have been shown to be involved in cleavage of plant chitinases. While fungal chitinases are responsible for cell wall remodeling during growth and morphogenesis, plant chitinases are important components of immunity. This study describes a dual function...
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Personal Name(s): | Ökmen, Bilal |
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Kemmerich, Bastian / Hilbig, Daniel / Wemhöner, Raphael / Aschenbroich, Jörn / Perrar, Andreas / Huesgen, Pitter / Schipper, Kerstin / Doehlemann, Gunther (Corresponding author) | |
Contributing Institute: |
Analytik; ZEA-3 |
Published in: | The new phytologist, 220 (2018) 1, S. 249 - 261 |
Imprint: |
Oxford [u.a.]
Wiley-Blackwell
2018
|
DOI: |
10.1111/nph.15265 |
PubMed ID: |
29916208 |
Document Type: |
Journal Article |
Research Program: |
Plant Science |
Subject (ZB): | |
Link: |
Restricted Published on 2018-06-19. Available in OpenAccess from 2019-06-19. Published on 2018-06-19. Available in OpenAccess from 2019-06-19. Restricted |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/19656 in citations.
Fungalysins from several phytopathogenic fungi have been shown to be involved in cleavage of plant chitinases. While fungal chitinases are responsible for cell wall remodeling during growth and morphogenesis, plant chitinases are important components of immunity. This study describes a dual function of the Ustilago maydis fungalysin UmFly1 in modulation of both plant and fungal chitinases. Genetic, biochemical and microscopic experiments were performed to elucidate the in vitro and in planta functions of U. maydis UmFly1. U. maydis ?umfly1 mutants show significantly reduced virulence, which coincides with reduced cleavage of the maize chitinase ZmChiA within its chitin-binding domain. Moreover, deletion of umfly1 affected the cell separation of haploid U. maydis sporidia. This phenotype is associated with posttranslational activation of the endogenous chitinase UmCts1. Genetic complementation of the ?umfly1 mutant with a homologous gene from closely related, but nonpathogenic, yeast fully rescued the cell separation defect in vitro, but it could not recover the ?umfly1 defect in virulence and cleavage of the maize chitinase. We report on the dual function of the secreted fungalysin UmFly1. We hypothesize that co-evolution of U. maydis with its host plant extended the endogenous function of UmFly1 towards the modulation of plant chitinase activity to promote infection. |