This title appears in the Scientific Report :
2018
Please use the identifier:
http://dx.doi.org/10.1021/acscatal.8b02935 in citations.
Enzyme-Polyelectrolyte Complexes Boost the Catalytic Performance of Enzymes
Enzyme-Polyelectrolyte Complexes Boost the Catalytic Performance of Enzymes
Understanding interactions between polymers and enzymes to boost enzymatic activity is of high importance for application of enzymes in multicomponent systems, such as laundry, food, pharmaceuticals, or cosmetics. Proteases are widely used in industries and increased performance in the presence of p...
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Personal Name(s): | J. Thiele, Martin |
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Davari, Mehdi D. / König, Melanie / Hofmann, Isabell / Junker, Niklas / Mirzaei Garakani, Tayebeh / Vojcic, Ljubica / Fitter, Jörg / Schwaneberg, Ulrich (Corresponding author) | |
Contributing Institute: |
Molekulare Biophysik; ICS-5 |
Published in: | ACS catalysis, 8 (2018) S. 10876–10887 |
Imprint: |
Washington, DC
ACS
2018
|
DOI: |
10.1021/acscatal.8b02935 |
Document Type: |
Journal Article |
Research Program: |
Functional Macromolecules and Complexes |
Publikationsportal JuSER |
Understanding interactions between polymers and enzymes to boost enzymatic activity is of high importance for application of enzymes in multicomponent systems, such as laundry, food, pharmaceuticals, or cosmetics. Proteases are widely used in industries and increased performance in the presence of polymers has been reported. Boosting of enzymes activity by polymers and understanding of the molecular principles is of high interest in biomedical and biotechnological applications. A molecular understanding of the boosting effect of poly(acrylic acid) (PAA) and poly(l-γ-glutamic acid) (γ-PGA) for a nonspecific subtilisin protease (Protein Database (PDB) ID: 1ST3) was generated through biophysical characterization (fluorescence correlation and circular dichroism spectroscopies, isothermal titration calorimetry), molecular dynamics simulations, and protease reengineering (site-saturation mutagenesis). Our study revealed that enthalpically driven interactions via key amino acid residues close to the protease Ca2+ binding sites cause the boosting effect in protease activity. On the molecular level electrostatic interactions results in the formation of protease-polyelectrolyte complexes. Site-saturation mutagenesis on positions S76, I77, A188, V238, N242, and K245 yielded an increased proteolytic performance against a complex protein mixture (trademark CO-3; up to ∼300% and ∼70%) in the presence of PAA and γ-PGA. Being able to fine-tune interactions between proteins and negatively charged polymers through integrative use of computational design, protein re-engineering and biophysical characterization proved to be an efficient workflow to improve protease performance. |