This title appears in the Scientific Report :
2018
Please use the identifier:
http://dx.doi.org/10.5562/cca3357 in citations.
Please use the identifier: http://hdl.handle.net/2128/19862 in citations.
Identification of stable and enantioselective lipases for biotechnological applications
Identification of stable and enantioselective lipases for biotechnological applications
The implementation of biocatalytic reactions in biotechnological processes requires enzymes with suitable properties. Thus, optimization of existing or identification of novel enzymes is needed. For enzyme optimization, various mutagenesis approaches are used yielding libraries with a large number o...
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Personal Name(s): | Jaeger, Karl-Erich |
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Fulton, Alexander / Schwaneberg, U. / Pietruszka, Jörg / Kovacic, Filip (Corresponding author) | |
Contributing Institute: |
Institut für Molekulare Enzymtechnologie (HHUD); IMET Institut für Bioorganische Chemie (HHUD); IBOC Biotechnologie; IBG-1 |
Published in: | Croatica chemica acta, 91 (2018) 2, S. 249-253 |
Imprint: |
Zagreb
2018
|
DOI: |
10.5562/cca3357 |
Document Type: |
Journal Article |
Research Program: |
Biotechnology |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/19862 in citations.
The implementation of biocatalytic reactions in biotechnological processes requires enzymes with suitable properties. Thus, optimization of existing or identification of novel enzymes is needed. For enzyme optimization, various mutagenesis approaches are used yielding libraries with a large number of protein variants. Additionally, novel enzymes can be identified from metagenomic libraries, which contain bacterial clones expressing DNA isolated from different habitats. Activity-based screening of such libraries is needed to identify enzyme candidates of interest. Here, we provide a protocol for screening of regio- and enantioselective lipases stable under industrially relevant conditions. |