This title appears in the Scientific Report :
2018
Please use the identifier:
http://dx.doi.org/10.1002/adbi.201800138 in citations.
Chip-Based Heat Stimulation for Modulating Signal Propagation in HL-1 Cell Networks
Chip-Based Heat Stimulation for Modulating Signal Propagation in HL-1 Cell Networks
Signal propagation in cardiac cell networks can be modulated by heat stimulation. Here, we investigate the response of a connected HL-1 cardiomyocyte cell network to the application of confined heat stimuli using Ca2+ imaging. Localized temperature gradients are generated by resistive heating via mi...
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Personal Name(s): | Dang, Ka My |
---|---|
Rinklin, Philipp / Afanasenkau, Dzmitry / Westmeyer, Gil / Schürholz, Tobias / Wiegand, Simone / Wolfrum, Bernhard (Corresponding author) | |
Contributing Institute: |
Weiche Materie; ICS-3 |
Published in: | Advanced biosystems, 2 (2018) S. 1800138 - |
Imprint: |
Weinheim
Wiley-VCH
2018
|
DOI: |
10.1002/adbi.201800138 |
Document Type: |
Journal Article |
Research Program: |
Functional Macromolecules and Complexes |
Publikationsportal JuSER |
Signal propagation in cardiac cell networks can be modulated by heat stimulation. Here, we investigate the response of a connected HL-1 cardiomyocyte cell network to the application of confined heat stimuli using Ca2+ imaging. Localized temperature gradients are generated by resistive heating via microwire arrays on a chip surface, which serves as a substrate for growing a confluent cell network. We demonstrate that upon heat stimulation, the velocity of the propagating Ca2+ wave in the network is locally increased, leading to a deformation of the wavefront. Furthermore, we show evidence of a change in the signal propagation direction caused by a relocation of the pacemaker cell. This effect might be used in future applications, where heat is employed as an alternative modality for cell stimulation protocols. |