This title appears in the Scientific Report :
2018
Chapter 10. Isotopically Enriched Systems
Chapter 10. Isotopically Enriched Systems
Most solid-state NMR measurements employ rare-spin nuclei, such as 13C or 15N, for detection. However, the low natural abundance of those spins limits the possibility of obtaining multidimensional homo- or hetero-nuclear solid-state NMR-spectra, which rely on internuclear correlations between those...
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Personal Name(s): | Hodgkinson, Paul (Editor) |
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Beumer, Claudia / Schölzel, Daniel / König, Anna / Uluca, Boran / Weirich, Franziska / Heise, Henrike (Corresponding author) | |
Contributing Institute: |
Strukturbiochemie; ICS-6 |
Published in: |
Modern methods in solid-state NMR : a practitioner's guide / Hodgkinson, Paul , London : Royal Society of Chemistry, 2018, |
Imprint: |
London
Royal Society of Chemistry
2018
|
Physical Description: |
xvi, 435 Seiten : illustrations |
ISBN: |
978-1-78801-046-7 9781788010467 9781788014199 |
Document Type: |
Contribution to a book |
Research Program: |
Functional Macromolecules and Complexes |
Series Title: |
New developments in NMR
No. 15 |
Subject (ZB): | |
Publikationsportal JuSER |
Most solid-state NMR measurements employ rare-spin nuclei, such as 13C or 15N, for detection. However, the low natural abundance of those spins limits the possibility of obtaining multidimensional homo- or hetero-nuclear solid-state NMR-spectra, which rely on internuclear correlations between those rare spins, unless signal enhancement or isotopic labelling is applied. In this chapter, we first give an overview of different techniques for selective and uniform isotope labelling of biomolecules. In the following sections, we describe different homo- and hetero-nuclear recoupling techniques and their use in multidimensional NMR spectroscopy. In particular, we emphasize the difference between zeroth-order recoupling techniques, which are well-suited for dipolar transfers between spins close in space, and second- and higher-order recoupling schemes, which allow the detection of long-range correlations. We also provide some examples how these techniques are applied towards structure elucidation of biomolecules. Finally, we briefly outline the technique of signal enhancement by dynamic nuclear polarization, a method that may in part help to overcome the need for isotopic enrichment. |