This title appears in the Scientific Report :
2019
Please use the identifier:
http://hdl.handle.net/2128/22501 in citations.
Please use the identifier: http://dx.doi.org/10.1038/s41598-019-45189-w in citations.
Structural Model of the ETR1 Ethylene Receptor Transmembrane Sensor Domain
Structural Model of the ETR1 Ethylene Receptor Transmembrane Sensor Domain
The structure, mechanism of action and copper stoichiometry of the transmembrane sensor domain of the plant ethylene receptor ETR1 and homologs have remained elusive, hampering the understanding on how the perception of the plant hormone ethylene is transformed into a downstream signal. We generated...
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Personal Name(s): | Schott-Verdugo, Stephan |
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Müller, Lena / Classen, Elisa / Gohlke, Holger (Corresponding author) / Groth, Georg (Corresponding author) | |
Contributing Institute: |
John von Neumann - Institut für Computing; NIC Jülich Supercomputing Center; JSC Strukturbiochemie; ICS-6 |
Published in: | Scientific reports, 9 (2019) 1, S. 8869 |
Imprint: |
[London]
Macmillan Publishers Limited, part of Springer Nature
2019
|
PubMed ID: |
31222090 |
DOI: |
10.1038/s41598-019-45189-w |
Document Type: |
Journal Article |
Research Program: |
Forschergruppe Gohlke Computational Science and Mathematical Methods |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1038/s41598-019-45189-w in citations.
The structure, mechanism of action and copper stoichiometry of the transmembrane sensor domain of the plant ethylene receptor ETR1 and homologs have remained elusive, hampering the understanding on how the perception of the plant hormone ethylene is transformed into a downstream signal. We generated the first structural model of the transmembrane sensor domain of ETR1 by integrating ab initio structure prediction and coevolutionary information. To refine and independently validate the model, we determined protein-related copper stoichiometries on purified receptor preparations and explored the helix arrangement by tryptophan scanning mutagenesis. All-atom molecular dynamics simulations of the dimeric model reveal how ethylene can bind proximal to the copper ions in the receptor, illustrating the initial stages of the ethylene perception process. |