This title appears in the Scientific Report :
2019
Please use the identifier:
http://dx.doi.org/10.1021/acs.bioconjchem.8b00874 in citations.
Anchor Peptide-Mediated Surface Immobilization of a Grubbs-Hoveyda-Type Catalyst for Ring-Opening Metathesis Polymerization
Anchor Peptide-Mediated Surface Immobilization of a Grubbs-Hoveyda-Type Catalyst for Ring-Opening Metathesis Polymerization
Adhesion promoting peptides have been reported to enable efficient enzyme immobilization on various material surfaces. Here we report the first immobilization of a synthetic Grubbs-Hoveyda (GH) type catalyst on two different materials (silica and polypropylene). To this end, the GH catalyst was coup...
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Personal Name(s): | Grimm, Alexander R. |
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Sauer, Daniel F. / Mirzaei Garakani, Tayebeh / Rübsam, Kristin / Polen, Tino / Davari, Mehdi D. / Jakob, Felix / Schiffels, Johannes / Okuda, Jun / Schwaneberg, Ulrich (Corresponding author) | |
Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | Bioconjugate chemistry, 30 (2019) 3, S. 714 - 720 |
Imprint: |
Columbus, Ohio
American Chemical Society
2019
|
DOI: |
10.1021/acs.bioconjchem.8b00874 |
PubMed ID: |
30642174 |
Document Type: |
Journal Article |
Research Program: |
Biotechnology |
Publikationsportal JuSER |
Adhesion promoting peptides have been reported to enable efficient enzyme immobilization on various material surfaces. Here we report the first immobilization of a synthetic Grubbs-Hoveyda (GH) type catalyst on two different materials (silica and polypropylene). To this end, the GH catalyst was coupled to an engineered (F16C) variant of the adhesion promoting peptide LCI through thiol-maleimide “click” reaction. Immobilization was performed in an oriented manner through the adhesion promoting peptide by simple incubation with the materials in water and subsequent washing with water and tetrahydrofuran. The immobilized GH catalyst was probed in ring-opening metathesis polymerization of a norbornene derivative to alter the surface properties in a layer-by-layer fashion. |