This title appears in the Scientific Report :
2019
Please use the identifier:
http://dx.doi.org/10.3390/ijms20184608 in citations.
Please use the identifier: http://hdl.handle.net/2128/23160 in citations.
Genetically encoded photosensitizers as light-triggered antimicrobial agents
Genetically encoded photosensitizers as light-triggered antimicrobial agents
Diseases caused by multi-drug resistant pathogens have become a global concern. Therefore, new approaches suitable for treating these bacteria are urgently needed. In this study, we analyzed genetically encoded photosensitizers (PS) related to the green fluorescent protein (GFP) or light-oxygen-volt...
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Personal Name(s): | Hilgers, Fabienne |
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Bitzenhofer, Nora Lisa / Ackermann, Yannic / Burmeister, Alina / Grünberger, Alexander / Jaeger, Karl-Erich / Drepper, Thomas (Corresponding author) | |
Contributing Institute: |
Institut für Molekulare Enzymtechnologie (HHUD); IMET Biotechnologie; IBG-1 |
Published in: | International journal of molecular sciences, 20 (2019) 18, S. 4608 - |
Imprint: |
Basel
Molecular Diversity Preservation International
2019
|
DOI: |
10.3390/ijms20184608 |
PubMed ID: |
31533368 |
Document Type: |
Journal Article |
Research Program: |
Biotechnology |
Link: |
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Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/23160 in citations.
Diseases caused by multi-drug resistant pathogens have become a global concern. Therefore, new approaches suitable for treating these bacteria are urgently needed. In this study, we analyzed genetically encoded photosensitizers (PS) related to the green fluorescent protein (GFP) or light-oxygen-voltage (LOV) photoreceptors for their exogenous applicability as light-triggered antimicrobial agents. Depending on their specific photophysical properties and photochemistry, these PSs can produce different toxic ROS (reactive oxygen species) such as O2•− and H2O2 via type-I, as well as 1O2 via type-II reaction in response to light. By using cell viability assays and microfluidics, we could demonstrate differences in the intracellular and extracellular phototoxicity of the applied PS. While intracellular expression and exogenous supply of GFP-related PSs resulted in a slow inactivation of E. coli and pathogenic Gram-negative and Gram-positive bacteria, illumination of LOV-based PSs such as the singlet oxygen photosensitizing protein SOPP3 resulted in a fast and homogeneous killing of these microbes. Furthermore, our data indicate that the ROS type and yield as well as the localization of the applied PS protein can strongly influence the antibacterial spectrum and efficacy. These findings open up new opportunities for photodynamic inactivation of pathogenic bacteria |