This title appears in the Scientific Report :
2020
Please use the identifier:
http://hdl.handle.net/2128/24433 in citations.
Please use the identifier: http://dx.doi.org/10.1111/1751-7915.13525 in citations.
An Ustilago maydis chassis for itaconic acid production without by‐products
An Ustilago maydis chassis for itaconic acid production without by‐products
Ustilago maydis is a promising yeast for the production of a range of valuable metabolites, including itaconate, malate, glycolipids and triacylglycerols. However, wild‐type strains generally produce a potpourri of all of these metabolites, which hinders efficient production of single target chemica...
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Personal Name(s): | Becker, Johanna |
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Hosseinpour Tehrani, Hamed / Gauert, Marc / Mampel, Jörg / Blank, Lars M. / Wierckx, Nick (Corresponding author) | |
Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | Microbial biotechnology, 13 (2020) 2, S. 350 - 362 |
Imprint: |
Oxford
Wiley-Blackwell
2020
|
DOI: |
10.1111/1751-7915.13525 |
PubMed ID: |
31880860 |
Document Type: |
Journal Article |
Research Program: |
Biotechnology |
Link: |
Get full text OpenAccess Get full text OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1111/1751-7915.13525 in citations.
Ustilago maydis is a promising yeast for the production of a range of valuable metabolites, including itaconate, malate, glycolipids and triacylglycerols. However, wild‐type strains generally produce a potpourri of all of these metabolites, which hinders efficient production of single target chemicals. In this study, the diverse by‐product spectrum of U. maydis was reduced through strain engineering using CRISPR/Cas9 and FLP/FRT, greatly increasing the metabolic flux into the targeted itaconate biosynthesis pathway. With this strategy, a marker‐free chassis strain could be engineered, which produces itaconate from glucose with significantly enhanced titre, rate and yield. The lack of by‐product formation not only benefited itaconate production, it also increases the efficiency of downstream processing improving cell handling and product purity. |