This title appears in the Scientific Report :
2021
Please use the identifier:
http://dx.doi.org/10.1093/molbev/msaa234 in citations.
Please use the identifier: http://hdl.handle.net/2128/30063 in citations.
Complex evolution of light-dependent protochlorophyllide oxidoreductases in aerobic anoxygenic phototrophs: origin, phylogeny and function
Complex evolution of light-dependent protochlorophyllide oxidoreductases in aerobic anoxygenic phototrophs: origin, phylogeny and function
Light-dependent and dark-operative protochlorophyllide oxidoreductases (LPORs and DPORs) are evolutionary and structurally distinct enzymes that are essential for the synthesis of (bacterio)chlorophyll, the primary pigment needed for both anoxygenic and oxygenic photosynthesis. In contrast to the lo...
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Personal Name(s): | Chernomor, Olga |
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Peters, Lena / Schneidewind, Judith / Loeschcke, Anita / Knieps-Grünhagen, Esther / Schmitz, Fabian / von Lieres, Eric / Kutta, Roger Jan / Svensson, Vera / Jaeger, Karl-Erich / Drepper, Thomas / von Haeseler, Arndt / Krauss, Ulrich (Corresponding author) | |
Contributing Institute: |
Biotechnologie; IBG-1 Institut für Molekulare Enzymtechnologie (HHUD); IMET |
Published in: | Molecular biology and evolution, 38 (2021) 3, S. 819-837 |
Imprint: |
Oxford
Oxford Univ. Press
2021
|
DOI: |
10.1093/molbev/msaa234 |
PubMed ID: |
32931580 |
Document Type: |
Journal Article |
Research Program: |
Biological and environmental resources for sustainable use |
Link: |
Get full text Get full text OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/30063 in citations.
Light-dependent and dark-operative protochlorophyllide oxidoreductases (LPORs and DPORs) are evolutionary and structurally distinct enzymes that are essential for the synthesis of (bacterio)chlorophyll, the primary pigment needed for both anoxygenic and oxygenic photosynthesis. In contrast to the long-held hypothesis that LPORs are only present in oxygenic phototrophs, we recently identified a functional LPOR in the aerobic anoxygenic phototrophic bacterium (AAPB) Dinoroseobacter shibae, and attributed its presence to a single horizontal gene transfer (HGT) event from cyanobacteria. Here, we provide evidence for the more widespread presence of genuine LPOR enzymes in AAPBs. An exhaustive bioinformatics search identified 36 putative LPORs outside of oxygenic phototrophic bacteria (cyanobacteria) with the majority being AAPBs. Using in vitro and in vivo assays, we show that the large majority of the tested AAPB enzymes are genuine LPORs. Solution structural analyses, performed for two of the AAPB LPORs, revealed a globally conserved structure when compared to a well-characterized cyanobacterial LPOR. Phylogenetic analyses suggest that LPORs were transferred not only from cyanobacteria, but also subsequently between proteobacteria and from proteobacteria to Gemmatimonadetes. Our study thus provides another interesting example for the complex evolutionary processes that govern the evolution of bacteria, involving multiple HGT events that likely occurred at different time points and involved different donors. |