This title appears in the Scientific Report :
2020
Please use the identifier:
http://dx.doi.org/10.1016/j.jbiotec.2020.09.016 in citations.
Please use the identifier: http://hdl.handle.net/2128/26006 in citations.
Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2,3-diol dehydrogenase
Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2,3-diol dehydrogenase
The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well st...
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Personal Name(s): | Muschallik, Lukas |
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Kipp, Carina Ronja / Recker, Inga / Bongaerts, Johannes / Pohl, Martina / Gellissen, Melanie / Schöning, Michael J. / Selmer, Thorsten / Siegert, Petra (Corresponding author) | |
Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | Journal of biotechnology, 324 (2020) S. 61 - 70 |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2020
|
DOI: |
10.1016/j.jbiotec.2020.09.016 |
Document Type: |
Journal Article |
Research Program: |
Biotechnology |
Link: |
Published on 2020-09-22. Available in OpenAccess from 2021-09-22. Published on 2020-09-22. Available in OpenAccess from 2021-09-22. |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/26006 in citations.
The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled. |