This title appears in the Scientific Report :
2020
Please use the identifier:
http://hdl.handle.net/2128/25892 in citations.
Please use the identifier: http://dx.doi.org/10.1101/2020.07.28.225011 in citations.
MAGIC: A label-free fluorescence method for 3D high-resolution reconstruction of myelinated fibers in large volumes
MAGIC: A label-free fluorescence method for 3D high-resolution reconstruction of myelinated fibers in large volumes
Analyzing the structure of neuronal fibers with single axon resolution, in large volumes, remains an unresolved challenge in connectomics. Here, we propose MAGIC (Myelin Autofluorescence imaging by Glycerol Induced Contrast enhancement), a simple tissue preparation method to perform label-free fluor...
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Personal Name(s): | Costantini, Irene (Corresponding author) |
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Baria, Enrico / Sorelli, Michele / Matuschke, Felix / Giardini, Francesco / Menzel, Miriam / Mazzamuto, Giacomo / Silvestri, Ludovico / Cicchi, Riccardo / Amunts, Katrin / Axer, Markus / Pavone, Francesco Saverio | |
Contributing Institute: |
Strukturelle und funktionelle Organisation des Gehirns; INM-1 |
Imprint: |
2020
|
DOI: |
10.1101/2020.07.28.225011 |
Document Type: |
Preprint |
Research Program: |
Human Brain Project Specific Grant Agreement 3 Human Brain Project Specific Grant Agreement 2 Connectivity and Activity |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1101/2020.07.28.225011 in citations.
Analyzing the structure of neuronal fibers with single axon resolution, in large volumes, remains an unresolved challenge in connectomics. Here, we propose MAGIC (Myelin Autofluorescence imaging by Glycerol Induced Contrast enhancement), a simple tissue preparation method to perform label-free fluorescence imaging of myelinated fibers. We demonstrate its broad applicability by performing mesoscopic reconstruction at sub-micron resolution of mouse, rat, monkey, and human brain samples and by quantifying the different fiber organization in Control and Reeler mouse’s hippocampal sections. |