This title appears in the Scientific Report :
2020
Please use the identifier:
http://hdl.handle.net/2128/25904 in citations.
Please use the identifier: http://dx.doi.org/10.3389/fbioe.2020.584614 in citations.
Revisiting the growth modulon of Corynebacterium glutamicum under glucose limited chemostat conditions
Revisiting the growth modulon of Corynebacterium glutamicum under glucose limited chemostat conditions
Increasing the growth rate of the industrial host Corynebacterium glutamicum is a promising target to rise productivities of growth coupled product formation. As a prerequisite, detailed knowledge about the tight regulation network is necessary for identifying promising metabolic engineering goals....
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Personal Name(s): | Graf, Michaela |
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Haas, Thorsten / Teleki, Attila / Feith, André / Cerff, Martin / Wiechert, Wolfgang / Nöh, Katharina / Busche, Tobias / Kalinowski, Jörn / Takors, Ralf (Corresponding author) | |
Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | Frontiers in Bioengineering and Biotechnology, 8 (2020) S. 584614 |
Imprint: |
Lausanne
Frontiers Media
2020
|
PubMed ID: |
33178676 |
DOI: |
10.3389/fbioe.2020.584614 |
Document Type: |
Journal Article |
Research Program: |
Biotechnology |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.3389/fbioe.2020.584614 in citations.
Increasing the growth rate of the industrial host Corynebacterium glutamicum is a promising target to rise productivities of growth coupled product formation. As a prerequisite, detailed knowledge about the tight regulation network is necessary for identifying promising metabolic engineering goals. Here, we present comprehensive metabolic and transcriptional analysis of C. glutamicum ATCC 13032 growing under glucose limited chemostat conditions with μ = 0.2, 0.3, and 0.4 h–1. Intermediates of central metabolism mostly showed rising pool sizes with increasing growth. 13C-metabolic flux analysis (13C-MFA) underlined the fundamental role of central metabolism for the supply of precursors, redox, and energy equivalents. Global, growth-associated, concerted transcriptional patterns were not detected giving rise to the conclusion that glycolysis, pentose-phosphate pathway, and citric acid cycle are predominately metabolically controlled under glucose-limiting chemostat conditions. However, evidence is found that transcriptional regulation takes control over glycolysis once glucose-rich growth conditions are installed. |