This title appears in the Scientific Report :
2020
Please use the identifier:
http://dx.doi.org/10.1134/S1027451020070046 in citations.
Please use the identifier: http://hdl.handle.net/2128/26042 in citations.
In Situ Dynamic Light Scattering Complementing Neutron Spin Echo Measurements on Protein Samples
In Situ Dynamic Light Scattering Complementing Neutron Spin Echo Measurements on Protein Samples
Monitoring the state of the sample on the minute-time scale is crucial in case of sensitive soft matter or biological samples, given that neutron spin echo measurements take up to several days. Moreover, there is no method to interpret the normalized intermediate scattering function obtained by neut...
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Personal Name(s): | Balacescu, L. |
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Vögl, F. / Staringer, S. / Ossovyi, V. / Brandl, G. / Lumma, N. / Feilbach, H. / Holderer, O. / Pasini, S. / Stadler, A. / Fitter, J. / Schrader, T. E. (Corresponding author) | |
Contributing Institute: |
Zelluläre Strukturbiologie; IBI-6 Heinz Maier-Leibnitz Zentrum; MLZ Neutronenstreuung; JCNS-1 JCNS-FRM-II; JCNS-FRM-II |
Published in: | Journal of surface investigation, 14 (2020) S1, S. S185 - S189 |
Imprint: |
Berlin
Springer Science+Business Media
2020
|
Physical Description: |
185-189 |
DOI: |
10.1134/S1027451020070046 |
Conference: | ECNS-European Neutron Scattering Conference, St. Petersburg (Russia), 2019-06-30 - 2019-07-05 |
Document Type: |
Contribution to a conference proceedings Journal Article |
Research Program: |
Soft Matter, Health and Life Sciences FRM II / MLZ Jülich Centre for Neutron Research (JCNS) |
Subject (ZB): | |
Link: |
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Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/26042 in citations.
Monitoring the state of the sample on the minute-time scale is crucial in case of sensitive soft matter or biological samples, given that neutron spin echo measurements take up to several days. Moreover, there is no method to interpret the normalized intermediate scattering function obtained by neutron spin echo measurements if relevant sample properties change during the measurement. Dynamic light scattering provides information on the diffusion constant of particles in solution (biological macromolecules like proteins, protein aggregates, polymer particles, etc.) with average hydrodynamic radii in a broad range from a few nanometers up to several microns. This information can be obtained within a few minutes and it offers a good overview of the current sample state. Details on the novel in situ dynamic light scattering set-up with one fixed scattering angle and first results obtained on a molten globule state of apo-myoglobin are presented. |