This title appears in the Scientific Report :
2020
Please use the identifier:
http://hdl.handle.net/2128/26010 in citations.
Please use the identifier: http://dx.doi.org/10.3390/ijms21218149 in citations.
Fluorescence Correlation Spectroscopy Reveals Interaction of Some Microdomain-Associated Lipids with Cellular Focal Adhesion Sites
Fluorescence Correlation Spectroscopy Reveals Interaction of Some Microdomain-Associated Lipids with Cellular Focal Adhesion Sites
Cells adhere to the extracellular matrix at distinct anchoring points, mostly focal adhesions. These are rich in immobile transmembrane- and cytoskeletal-associated proteins, some of which are known to interact with lipids of the plasma membrane. To investigate their effect on lipid mobility and mol...
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Personal Name(s): | Kleusch, Christian |
---|---|
Monzel, Cornelia / Sridhar, Krishna Chander / Hoffmann, Bernd / Csiszár, Agnes / Merkel, Rudolf (Corresponding author) | |
Contributing Institute: |
Mechanobiologie; IBI-2 |
Published in: | International journal of molecular sciences, 21 (2020) 21, S. 8149 - |
Imprint: |
Basel
Molecular Diversity Preservation International
2020
|
PubMed ID: |
33142729 |
DOI: |
10.3390/ijms21218149 |
Document Type: |
Journal Article |
Research Program: |
Engineering Cell Function |
Link: |
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Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.3390/ijms21218149 in citations.
Cells adhere to the extracellular matrix at distinct anchoring points, mostly focal adhesions. These are rich in immobile transmembrane- and cytoskeletal-associated proteins, some of which are known to interact with lipids of the plasma membrane. To investigate their effect on lipid mobility and molecular interactions, fluorescently labeled lipids were incorporated into the plasma membranes of primary myofibroblasts using fusogenic liposomes. With fluorescence correlation spectroscopy, we tested mobilities of labeled microdomain-associated lipids such as sphingomyelin (SM), ganglioside (GM1), and cholesterol as well as of a microdomain-excluded phospholipid (PC) and a lipid-like molecule (DiIC18(7)) in focal adhesions (FAs) and in neighboring non-adherent membrane areas. We found significantly slower diffusion of SM and GM1 inside FAs but no effect on cholesterol, PC, and DiIC18(7). These data were compared to the molecular behavior in Lo/Ld-phase separated giant unilamellar vesicles, which served as a model system for microdomain containing lipid membranes. In contrast to the model system, lipid mobility changes in FAs were molecularly selective, and no particle enrichment occurred. Our findings suggest that lipid behavior in FAs cannot be described by Lo/Ld-phase separation. The observed slow-down of some molecules in FAs is potentially due to transient binding between lipids and some molecular constituent(s). |