This title appears in the Scientific Report :
2020
Please use the identifier:
http://dx.doi.org/10.7554/eLife.50027 in citations.
Please use the identifier: http://hdl.handle.net/2128/26618 in citations.
The guide sRNA sequence determines the activity level of box C/D RNPs
The guide sRNA sequence determines the activity level of box C/D RNPs
2’-O-rRNA methylation, which is essential in eukaryotes and archaea, is catalysed by the Box C/D RNP complex in an RNA-guided manner. Despite the conservation of the methylation sites, the abundance of site-specific modifications shows variability across species and tissues, suggesting that rRNA met...
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Personal Name(s): | Graziadei, Andrea |
---|---|
Gabel, Frank / Kirkpatrick, John / Carlomagno, Teresa (Corresponding author) | |
Contributing Institute: |
Heinz Maier-Leibnitz Zentrum; MLZ JCNS-FRM-II; JCNS-FRM-II |
Published in: | eLife, 9 (2020) S. e50027 |
Imprint: |
Cambridge
eLife Sciences Publications
2020
|
DOI: |
10.7554/eLife.50027 |
Document Type: |
Journal Article |
Research Program: |
FRM II / MLZ Jülich Centre for Neutron Research (JCNS) |
Subject (ZB): | |
Link: |
OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/26618 in citations.
2’-O-rRNA methylation, which is essential in eukaryotes and archaea, is catalysed by the Box C/D RNP complex in an RNA-guided manner. Despite the conservation of the methylation sites, the abundance of site-specific modifications shows variability across species and tissues, suggesting that rRNA methylation may provide a means of controlling gene expression. As all Box C/D RNPs are thought to adopt a similar structure, it remains unclear how the methylation efficiency is regulated. Here, we provide the first structural evidence that, in the context of the Box C/D RNP, the affinity of the catalytic module fibrillarin for the substrate–guide helix is dependent on the RNA sequence outside the methylation site, thus providing a mechanism by which both the substrate and guide RNA sequences determine the degree of methylation. To reach this result, we develop an iterative structure-calculation protocol that exploits the power of integrative structural biology to characterize conformational ensembles. |