This title appears in the Scientific Report :
2020
Please use the identifier:
http://hdl.handle.net/2128/26796 in citations.
Please use the identifier: http://dx.doi.org/10.1107/S2053230X19002851 in citations.
Igni18, a novel metallo-hydrolase from the hyperthermophilic archaeon Ignicoccus hospitalis KIN4/I: cloning, expression, purification and X-ray analysis
Igni18, a novel metallo-hydrolase from the hyperthermophilic archaeon Ignicoccus hospitalis KIN4/I: cloning, expression, purification and X-ray analysis
The hyperthermophilic crenarchaeon Ignicoccus hospitalis KIN4/I possesses at least 35 putative genes encoding enzymes that belong to the α/β-hydrolase superfamily. One of those genes, the metallo-hydrolase-encoding igni18, was cloned and heterologously expressed in Pichia pastoris. The enzyme produc...
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Personal Name(s): | Kobus, Stefanie (Corresponding author) |
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Perez-Garcia, Pablo / Hoeppner, Astrid / Holzscheck, Nicholas / Kovacic, Filip / Streit, Wolfgang R. / Jaeger, Karl-Erich / Chow, Jennifer / Smits, Sander H. J. | |
Contributing Institute: |
Institut für Molekulare Enzymtechnologie (HHUD); IMET |
Published in: | Acta crystallographica / F, 75 (2019) 4, S. 307 - 311 |
Imprint: |
Oxford [u.a.]
Blackwell
2019
|
DOI: |
10.1107/S2053230X19002851 |
Document Type: |
Journal Article |
Research Program: |
Biotechnology |
Link: |
OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1107/S2053230X19002851 in citations.
The hyperthermophilic crenarchaeon Ignicoccus hospitalis KIN4/I possesses at least 35 putative genes encoding enzymes that belong to the α/β-hydrolase superfamily. One of those genes, the metallo-hydrolase-encoding igni18, was cloned and heterologously expressed in Pichia pastoris. The enzyme produced was purified in its catalytically active form. The recombinant enzyme was successfully crystallized and the crystal diffracted to a resolution of 2.3 Å. The crystal belonged to space group R32, with unit-cell parameters a = b = 67.42, c = 253.77 Å, α = β = 90.0, γ = 120.0°. It is suggested that it contains one monomer of Igni18 within the asymmetric unit. |