This title appears in the Scientific Report :
2020
Please use the identifier:
http://hdl.handle.net/2128/27017 in citations.
Please use the identifier: http://dx.doi.org/10.1021/acs.jpclett.9b03798 in citations.
All-Atom Simulations Disclose How Cytochrome Reductase Reshapes the Substrate Access/Egress Routes of Its Partner CYP450s
All-Atom Simulations Disclose How Cytochrome Reductase Reshapes the Substrate Access/Egress Routes of Its Partner CYP450s
Cytochromes P450 enzymes (CYP450s) promote the oxidative metabolism of a variety of substrates via the electrons supplied by the cytochrome P450 reductase (CPR) and upon formation of a CPR/CYP450 adduct. In spite of the pivotal regulatory importance of this process, the impact of CPR binding on the...
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Personal Name(s): | Ritacco, Ida |
---|---|
Saltalamacchia, Andrea / Spinello, Angelo / Ippoliti, Emiliano / Magistrato, Alessandra (Corresponding author) | |
Contributing Institute: |
JARA - HPC; JARA-HPC Computational Biomedicine; INM-9 Computational Biomedicine; IAS-5 |
Published in: | The journal of physical chemistry letters, 11 (2020) 4, S. 1189 - 1193 |
Imprint: |
Washington, DC
ACS
2020
|
DOI: |
10.1021/acs.jpclett.9b03798 |
Document Type: |
Journal Article |
Research Program: |
Post-Transcriptional regulation mechanism of Human Aromatase investigated by molecular simulations Addenda |
Link: |
Published on 2020-01-27. Available in OpenAccess from 2021-01-27. Restricted |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1021/acs.jpclett.9b03798 in citations.
Cytochromes P450 enzymes (CYP450s) promote the oxidative metabolism of a variety of substrates via the electrons supplied by the cytochrome P450 reductase (CPR) and upon formation of a CPR/CYP450 adduct. In spite of the pivotal regulatory importance of this process, the impact of CPR binding on the functional properties of its partner CYP450 remains elusive. By performing multiple microsecond-long all-atom molecular dynamics simulations of a 520 000-atom model of a CPR/CYP450 adduct embedded in a membrane mimic, we disclose the molecular terms for their interactions, considering the aromatase (HA) enzyme as a proxy of the CYP450 family. Our study strikingly unveils that CPR binding alters HA’s functional motions, bolstering a change in the shape and type of the channels traveled by substrates/products during their access/egress to/from the enzyme’s active site. Our outcomes unprecedentedly contribute to extricate the many entangled facets of the CYP450 metabolon, redrafting its intricate panorama from an atomic-level perspective. |