This title appears in the Scientific Report :
2021
Please use the identifier:
http://hdl.handle.net/2128/27561 in citations.
The ability of arbuscular mycorrhizal fungi to transport iron from poorly soluble compounds into spring barley (Hordeum vulgare)
The ability of arbuscular mycorrhizal fungi to transport iron from poorly soluble compounds into spring barley (Hordeum vulgare)
Arbuscular mycorrhizal fungi can have symbiotic interactions with around 80 % of land plant species which allows an alternative nutrient uptake pathway into plants. Depending on various factors, this leads to increased uptake of nutrients by the plant in nutrient-poor soils or to a more controlled u...
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Personal Name(s): | Beißmann, Judith (Corresponding author) |
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Berns, Anne E. (Thesis advisor) / Klumpp, Erwin (Reviewer) / Schäffer, Andreas (Reviewer) | |
Contributing Institute: |
Agrosphäre; IBG-3 |
Imprint: |
2021
|
Physical Description: |
78 |
Dissertation Note: |
Masterarbeit, RWTH Aachen, 2021 |
Document Type: |
Master Thesis |
Research Program: |
Agro-biogeosystems: controls, feedbacks and impact Für eine nachhaltige Bio-Ökonomie – von Ressourcen zu Produkten |
Link: |
OpenAccess |
Publikationsportal JuSER |
Arbuscular mycorrhizal fungi can have symbiotic interactions with around 80 % of land plant species which allows an alternative nutrient uptake pathway into plants. Depending on various factors, this leads to increased uptake of nutrients by the plant in nutrient-poor soils or to a more controlled uptake in nutrient-rich soils. Several studies have investigated the uptake of iron from the soil into the plant in symbiosis with AMF. However, it is still not clarified that poorly soluble iron compounds can be mobilized by AMF and the uptake into plants. Iron in the soil is mainly found in poorly soluble compounds such as Fe-(III) hydroxide, oxyhydroxides, oxides or silicates which cannot be taken up by plants and have to be mobilized first.The present study focuses on the influence and effects of AMF on iron uptake into spring barley and whether AMF can mobilize iron out of poorly soluble Fe(hydr-)oxides and support the uptake. Aiming for this, a pot experiment with spring barley and a Glomeraceae mixture was conducted growing in an agricultural soil. To focus on the effect of AMF, a tube was prepared which in theory, only AMF hyphae were able to access. This tube was filled with soil containing labeled 57Fe in form of Fe-(hydr)oxide and placed in each pot between two plants.The dry weights of the roots decreased from booting to flowering to dead-ripe whereby +AMF plants had highly significantly lower root dry weights during flowering and dead-ripe in contrast to the controls. Also, the leaves showed significantly lower dry weights in +AMF plants at dead-ripe stage. In addition, a difference in the water uptake of the plants was evident at the end of ripening. The +AMF plants needed significantly less water than the controls. Differences in the iron content were observed in the stems and senescent leaves of the controls during flowering showing higher concentrations. Although the spiked soil had an iron isotopic composition (δ57Febulk) of around 250 ‰ and a δ57FeHCl of 1045 ‰, the 57Fe did not show up in the plants. Only one replicate showed a positive shift with a heavier iron isotopic composition in leaves, stems, and ears, whereas the roots were isotopically lighter. These samples were from a pot with AMF, but closer inspection showed that root exclusion did not work so that probably root hairs penetrated into the tube. It cannot be excluded that the positive shift as well as the large negative shift in the other samples were due to mass interferences from matrix elements, polyatomic interferences, and the high uncertainty from the measurement itself. Due to the missing AMF analysis, it could only be assumed that the AMF colonization intensity was low. The fact that AMF inoculation worked could be assessed by several observations such as the difference between the root dry weights and the reduced water demand during ripening. The latter was probably because AMF leads to faster plant growth under high temperatures. Barley is difficult to colonize and tends to have only a low AMF colonization intensity in contrast to other plant species. Summarized, the aims of the study could not be achieved or, due to the small number of samples, it could not be confirmed or rejected that AMF can contribute to the dissolution of iron from poorly soluble compounds and transport into the plant. |