This title appears in the Scientific Report :
2021
Please use the identifier:
http://dx.doi.org/10.1021/acs.langmuir.1c00981 in citations.
Please use the identifier: http://hdl.handle.net/2128/28360 in citations.
Structural Organization of Cardiolipin-Containing Vesicles as Models of the Bacterial Cytoplasmic Membrane
Structural Organization of Cardiolipin-Containing Vesicles as Models of the Bacterial Cytoplasmic Membrane
The bacterial cytoplasmic membrane is the innermost bacterial membrane and is mainly composed of three different phospholipid species, i.e., phosphoethanolamine (PE), phosphoglycerol (PG), and cardiolipin (CL). In particular, PG and CL are responsible for the negative charge of the membrane and are...
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Personal Name(s): | Luchini, Alessandra (Corresponding author) |
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Cavasso, Domenico / Radulescu, Aurel / D’Errico, Gerardino / Paduano, Luigi / Vitiello, Giuseppe (Corresponding author) | |
Contributing Institute: |
Neutronenstreuung; JCNS-1 JCNS-4; JCNS-4 Heinz Maier-Leibnitz Zentrum; MLZ JCNS-FRM-II; JCNS-FRM-II |
Published in: | Langmuir, 37 (2021) 28, S. 8508 - 8516 |
Imprint: |
Washington, DC
ACS Publ.
2021
|
DOI: |
10.1021/acs.langmuir.1c00981 |
Document Type: |
Journal Article |
Research Program: |
Materials – Quantum, Complex and Functional Materials Jülich Centre for Neutron Research (JCNS) (FZJ) |
Subject (ZB): | |
Link: |
Published on 2021-07-02. Available in OpenAccess from 2022-07-02. Restricted |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/28360 in citations.
The bacterial cytoplasmic membrane is the innermost bacterial membrane and is mainly composed of three different phospholipid species, i.e., phosphoethanolamine (PE), phosphoglycerol (PG), and cardiolipin (CL). In particular, PG and CL are responsible for the negative charge of the membrane and are often the targets of cationic antimicrobial agents. The growing resistance of bacteria toward the available antibiotics requires the development of new and more efficient antibacterial drugs. In this context, studying the physicochemical properties of the bacterial cytoplasmic membrane is pivotal for understanding drug–membrane interactions at the molecular level as well as for designing drug-testing platforms. Here, we discuss the preparation and characterization of PE/PG/CL vesicle suspensions, which contain all of the main lipid components of the bacterial cytoplasmic membrane. The vesicle suspensions were characterized by means of small-angle neutron scattering, dynamic light scattering, and electron paramagnetic spectroscopy. By combining solution scattering and spectroscopy techniques, we propose a detailed description of the impact of different CL concentrations on the structure and dynamics of the PE/PG bilayer. CL induces the formation of thicker bilayers, which exhibit higher curvature and are overall more fluid. The experimental results contribute to shed light on the structure and dynamics of relevant model systems of the bacterial cytoplasmic membrane. |