This title appears in the Scientific Report :
2021
Please use the identifier:
http://dx.doi.org/10.1016/j.jcrysgro.2021.126362 in citations.
Please use the identifier: http://hdl.handle.net/2128/29452 in citations.
Real-time investigation of Lysozyme crystallization kinetics: A neutron diffraction study
Real-time investigation of Lysozyme crystallization kinetics: A neutron diffraction study
The isothermal crystallization kinetics of hen-egg-white Lysozyme has been investigatedby means of a time-resolved neutron diffraction experiment for almost 3 days,starting from a supersaturated solution of Lysozyme (30 mg/ml, 3 wt % NaCl, pD 4.75at 298 K) until the growth of crystals, in order to h...
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Personal Name(s): | Longo, M. |
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Heigl, R. J. / Schrader, Tobias Erich (Corresponding author) | |
Contributing Institute: |
Neutronenstreuung; JCNS-1 Heinz Maier-Leibnitz Zentrum; MLZ JCNS-4; JCNS-4 JCNS-FRM-II; JCNS-FRM-II |
Published in: | Journal of crystal growth, 576 (2021) S. 126362 |
Imprint: |
Amsterdam [u.a.]
Elsevier
2021
|
DOI: |
10.1016/j.jcrysgro.2021.126362 |
Document Type: |
Journal Article |
Research Program: |
Materials – Quantum, Complex and Functional Materials Jülich Centre for Neutron Research (JCNS) (FZJ) |
Subject (ZB): | |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/29452 in citations.
The isothermal crystallization kinetics of hen-egg-white Lysozyme has been investigatedby means of a time-resolved neutron diffraction experiment for almost 3 days,starting from a supersaturated solution of Lysozyme (30 mg/ml, 3 wt % NaCl, pD 4.75at 298 K) until the growth of crystals, in order to have complementary informationabout the crystallization kinetics. The temporal evolution of the intensity of the Braggpeaks, observed in the neutron diffraction images when a single crystal appears, hasbeen studied. Simultaneously, the analysis of the small angle neutron scattering curvesfrom the Lysozyme solution, during the crystallization process, has been performed.A correlated behaviour between the decrease of the Lysozyme concentration and theincrease of the crystallization fraction was observed. The crystallization kinetics wasdescribed by means of the Johnson-Mehl-Avrami-Kolmogorov model and parameterscompatible with our previous Lysozyme crystallization study have been found.The Lysozyme crystallization under constant neutron flux underlines the known strengthof neutrons in studying biological samples without causing radiation damage. |