This title appears in the Scientific Report :
2022
Please use the identifier:
http://hdl.handle.net/2128/33175 in citations.
Please use the identifier: http://dx.doi.org/10.1007/s00604-021-04746-9 in citations.
Randomly positioned gold nanoparticles as fluorescence enhancers in apta-immunosensor for malaria test
Randomly positioned gold nanoparticles as fluorescence enhancers in apta-immunosensor for malaria test
A plasmon-enhanced fluorescence-based antibody-aptamer biosensor — consisting of gold nanoparticles randomly immobilized onto a glass substrate via electrostatic self-assembly — is described for specific detection of proteins in whole blood. Analyte recognition is realized through a sandwich scheme...
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Personal Name(s): | Minopoli, Antonio |
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Della Ventura, Bartolomeo / Campanile, Raffaele / Tanner, Julian A. / Offenhäusser, Andreas / Mayer, Dirk (Corresponding author) / Velotta, Raffaele | |
Contributing Institute: |
Bioelektronik; IBI-3 |
Published in: | Microchimica acta, 188 (2021) 3, S. 88 |
Imprint: |
Wien [u.a.]
Springer
2021
|
DOI: |
10.1007/s00604-021-04746-9 |
Document Type: |
Journal Article |
Research Program: |
Molecular Information Processing in Cellular Systems |
Link: |
OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1007/s00604-021-04746-9 in citations.
A plasmon-enhanced fluorescence-based antibody-aptamer biosensor — consisting of gold nanoparticles randomly immobilized onto a glass substrate via electrostatic self-assembly — is described for specific detection of proteins in whole blood. Analyte recognition is realized through a sandwich scheme with a capture bioreceptor layer of antibodies — covalently immobilized onto the gold nanoparticle surface in upright orientation and close-packed configuration by photochemical immobilization technique (PIT) — and a top bioreceptor layer of fluorescently labelled aptamers. Such a sandwich configuration warrants not only extremely high specificity, but also an ideal fluorophore-nanostructure distance (approximately 10–15 nm) for achieving strong fluorescence amplification. For a specific application, we tested the biosensor performance in a case study for the detection of malaria-related marker Plasmodium falciparum lactate dehydrogenase (PfLDH). The proposed biosensor can specifically detect PfLDH in spiked whole blood down to 10 pM (0.3 ng/mL) without any sample pretreatment. The combination of simple and scalable fabrication, potentially high-throughput analysis, and excellent sensing performance provides a new approach to biosensing with significant advantages compared to conventional fluorescence immunoassays |