This title appears in the Scientific Report :
2021
Please use the identifier:
http://dx.doi.org/10.1021/jacs.1c03409 in citations.
Please use the identifier: http://hdl.handle.net/2128/30440 in citations.
Real-Time Tracking of Proton Transfer from the Reactive Cysteine to the Flavin Chromophore of a Photosensing Light Oxygen Voltage Protein
Real-Time Tracking of Proton Transfer from the Reactive Cysteine to the Flavin Chromophore of a Photosensing Light Oxygen Voltage Protein
LOV (light oxygen voltage) proteins are photosensors ubiquitous to all domains of life. A variant of the short LOV protein from Dinoroseobacter shibae (DsLOV) exhibits an exceptionally fast photocycle. We performed time-resolved molecular spectroscopy on DsLOV-M49S and characterized the formation of...
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Personal Name(s): | Maia, Raiza N. A. |
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Ehrenberg, David / Oldemeyer, Sabine / Knieps-Grünhagen, Esther / Krauss, Ulrich / Heberle, Joachim (Corresponding author) | |
Contributing Institute: |
Institut für Molekulare Enzymtechnologie (HHUD); IMET Biotechnologie; IBG-1 |
Published in: | Journal of the American Chemical Society, 143 (2021) 32, S. 12535 - 12542 |
Imprint: |
Washington, DC
American Chemical Society
2021
|
PubMed ID: |
34347468 |
DOI: |
10.1021/jacs.1c03409 |
Document Type: |
Journal Article |
Research Program: |
Biological and environmental resources for sustainable use |
Link: |
Get full text Published on 2021-08-04. Available in OpenAccess from 2022-08-04. |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/30440 in citations.
LOV (light oxygen voltage) proteins are photosensors ubiquitous to all domains of life. A variant of the short LOV protein from Dinoroseobacter shibae (DsLOV) exhibits an exceptionally fast photocycle. We performed time-resolved molecular spectroscopy on DsLOV-M49S and characterized the formation of the thio-adduct state with a covalent bond between the reactive cysteine (C72) and C4a of the FMN. By use of a tunable quantum cascade laser, the weak absorption change of the vibrational band of S–H stretching vibration of C57 was resolved with a time resolution of 10 ns. Deprotonation of C72 proceeded with a time constant of 12 μs which tallies the rise of the thio-adduct state. These results provide valuable information for the mechanistic interpretation of light-induced structural changes in LOV domains, which involves the choreographed sequence of proton transfers, changes in electron density distributions, spin alterations of the latter, and transient bond formation and breakage. Such molecular insight will help develop new optogenetic tools based on flavin photoreceptors. |