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Bio-Carrier Vectors [E-Book] : Methods and Protocols /
Table of Contents: ... Nanoparticles as a Carrier of dsRNA for Gene Therapy -- Generation of Functional Insulin-Producing Cells from Mouse Embryonic Stem Cells through Protein Transduction of Transcription Factors -- Cardiac Targeting Peptide: From Identification to Validation to Mechanism...

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Wound Regeneration [E-Book] : Methods and Protocols  /
Table of Contents: ... of Dental Pulp-Derived Stem Cell Product for Healing -- Musculoskeletal Tissue Engineering Using Fibrous Biomaterials -- Cutaneous Wound Generation in Diabetic NOD/SCID Mice and the Use of Nanofiber-Expanded Hematopoietic Stem Cell Therapy -- Contusion Rodent...

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Channelrhodopsin [E-Book] : Methods and Protocols  /
Table of Contents: ... Interactions in Pups and Adults -- Chronic Optogenetic Pacing of Human Induced Pluripotent Stem Cell-Derived Engineered Cardiac Tissues -- Patterned Optogenetic Stimulation Using a DMD-Projector -- Selecting Channelrhodopsin Constructs for Optimal Visual Restoration...

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T-Cell Receptor Signaling [E-Book] : Methods and Protocols  /
Table of Contents: ... -- Characterization of Immune Cell Subset Expansion in Response to Therapeutic Treatment in Mice -- Primary T Cell Transduction to Study Follicular Helper T Cell Differentiation -- In vitro Generation of Stem Cell Memory-like T Cells from Activated T Cells -- Artificial...

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Retinal Development [E-Book] : Methods and Protocols  /
Table of Contents: ... in Retinal Morphological and Communication Studies -- Multi-electrode Array Recording of Mouse Retinas Transplanted with Stem-cell Derived Retinal Sheets -- Paired Recording to Study Electrical Coupling between Photoreceptors in Mouse Retina. ....

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Basophils and Mast Cells [E-Book] : Methods and Protocols /
Table of Contents: ... Mast Cell Phenotype from CD133+ Stem Cells -- Generation and Culture of Peripheral CD34+ Stem Cell-derived Mast Cells (PSCMCs) -- Modulating the Human Basophil Phenotype During its Development and Maturation: Basophils Derived from in vitro Cultures of CD34...

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Nucleic Acid Detection and Structural Investigations [E-Book] : Methods and Protocols  /
Table of Contents: ... Muscle Injury by Electroporation - A Model to Study Degeneration/Regeneration Pathways in Muscle -- Isolation and Characterization of Muscle Derived Stem Cells from Dystrophic Mouse Models -- Universal Library Preparation Protocol for Efficient High-Throughput...

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In Vitro Differentiation of T-Cells [E-Book] : Methods and Protocols  /
Table of Contents: ...-Specific Receptors -- In Vitro Conversion of Activated T-Cells into Stem Cell Memory-Like T-Cells -- Human iPSC Generation from Antigen-Specific T-Cells -- In Vitro Differentiation of T-Cells: From Human Embryonic Stem Cells and Induced Pluripotent Stem Cells...

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Metabolomics [E-Book] : Methods and Protocols  /
Table of Contents: ... and Quantification of Lipid Droplets in Differentiated Human Podocytes -- Labeling Cancer Stem Cells with 13C6 Glucose and 13C5 Glutamine for Metabolic Flux Analysis -- Protocols for Metabolomic Analyses from Tissues in Parkinson's Disease -- Application of Stable Isotope...

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Nuclear Receptors : Methods and Experimental Protocols [E-Book] /
Table of Contents: ... Embryonic Stem Cells through Nuclear Receptor Signaling -- Obtaining Crystals of PPAR Ligand Binding Domain Bound to Small Molecules -- Molecular Modeling Approach to Study the PPAR-Ligand Interactions -- High Dimensional Data Approaches to Understanding Nuclear...

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High Content Screening [E-Book] : A Powerful Approach to Systems Cell Biology and Phenotypic Drug Discovery /
Table of Contents: ... for Drug Discovery in 384-well Ultra-Low Attachment Microplates -- An Endothelial Cell/Mesenchymal Stem Cell Co-Culture Cord Formation Assay to Model Vascular Biology In Vitro -- High-throughput Automated Chemical Screens in Zebrafish....

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Glioblastoma [E-Book] : Methods and Protocols /
Table of Contents: ... -- Establishing Primary Human Glioblastoma Tumorsphere Cultures from Operative Specimens -- Isolation of GBM Stem Cells with Flow Cytometry -- Lentiviral Transduction of Primary Human Glioblastoma Cultures -- Selective Targeting of CD133-expressing Glioblastoma Stem...

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Intravital Imaging of Dynamic Bone and Immune Systems [E-Book] : Methods and Protocols /
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Biological Networks and Pathway Analysis [E-Book] /
Table of Contents: ...-Throughput Data to Understanding Causative Mechanisms for a Complex Disease -- Pharmacologic Manipulation of Wnt Signaling and Cancer Stem Cells -- Functional Network Disruptions in Schizophrenia....

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Synapse Development [E-Book] : Methods and Protocols /
Table of Contents: ... Using Neurons Cultured on Micropatterned Substrates -- Monitoring Synapses Via Trans-Synaptic GFP Complementation -- Generation of Spinal Motor Neurons from Human Pluripotent Stem Cells -- Biochemical Purification of Binding Partners of Synaptic Scaffold...

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