Table of Contents: “...Methods for the Isolation and Recovery of Bifidobacteria -- Bifidobacterium Transformation -- Isolation of Chromosomal and Plasmid DNA from Bifidobacteria -- Assembly, Annotation, and Comparative Analysis of Bifidobacterial Genomes -- Site-directed...”

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Table of Contents: “... Chromosomes in Individual Bacteria to Quantify their Impacts on Persistence -- Analyzing PERSISTER PROTEOMES with SILAC and Label-Free Methods -- Defining Proteomic Signatures To Predict Multi-Drug Persistence in Pseudomonas aeruginosa -- The use of Experimental...”

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Table of Contents: “... Resistant Staphylococcus aureus -- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA -- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains -- Spa Genotyping of Staphylococcus aureus Isolates -- Multilocus Sequence Typing...”

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Table of Contents: “...-Derived Chlamydia Using Laser Microdissection -- Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions -- Mutagenesis of Chlamydia trachomatis Using TargeTron -- Forward and Reverse Genetic Analysis of Chlamydia -- A Co-Infection Model...”

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Table of Contents: “... in Salmonella spp. -- Red-mediated Recombineering of Salmonella enterica Genomes -- A Method to Introduce an Internal Tag Sequence into a Salmonella Chromosomal Gene -- Generation and use of Site-directed Chromosomal cyaA’ Translational Fusions in Salmonella enteric...”

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Table of Contents: “... -- Multilocus Sequence Typing (MLST) of Staphylococcus aureus -- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA -- Genetic Interruption of Target Genes for Investigation of Virulence Factors -- Molecular Analysis of Staphylococcal Superantigens...”

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Table of Contents: “... by RNA Sequencing -- Gene Amplification and qRT-PCR -- The Standard European Vector architecture (SEVA) plasmid toolkit -- Chromosomal integration of transcriptional fusions -- A Method to Capture Large DNA Fragments from Genomic DNA -- Transposon Mutagenesis...”

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