Table of Contents: “...Methods to Visualize the Actin Cytoskeleton during Plant Cell Division -- Immunolabeling of Nuclei/Chromosomes in Arabidopsis thaliana -- A Protocol for Genome-Wide Analysis of DNA Replication Timing in Intact Root Tips -- Assaying Cyclin-Dependent...”

Full text 

Table of Contents: “...A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis -- Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes -- How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis...”

Full text 

Table of Contents: “... -- Sequencing and Assembling Genomes and Chromosomes of Cereal Crops -- Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing -- Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR...”

Full text 

Table of Contents: “... -- Section Overview: Unravelling the Complex Epigenetic Mechanisms that Regulate Gene Activity -- Technical Review: A Hitchhikers Guide to Chromosome Conformation Capture -- 3C in Maize and Arabidopsis -- Profiling Histone Modifications in Synchronised Floral...”

Full text 

Table of Contents: “... Lines for QTL Mapping -- Assessing Distribution and Variation of Genome-wide DNA Methylation using Short Read Sequencing -- Circular Chromosome Conformation Capture in Plants -- Genome Wide Profiling of Histone Modifications and Histone Variants...”

Full text 

Table of Contents: “... Spreading and Image Quality for the Study of Chromosomes in Plant Tissues -- Whole Mount RNA-FISH on Ovules and Developing Seeds -- Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy -- Transmission Electron...”

Full text 

Table of Contents: “... with Cell Cycle Inhibitors -- Isolation of Plant Nuclei at Defined Cell Cycle Stages Using Edu Labeling and Flow Cytometry -- Preparation and Fluorescent Analysis of Plant Metaphase Chromosomes -- Fluorescence In Situ Hybridization On Rice Chromosomes -- Dynamics...”

Full text 

Table of Contents: “...C-Banding of Plant Chromosomes -- Chromosome Painting by GISH and Multi-Color FISH -- Fluorescent In Situ Hybridization on Extended Chromatin Fibers for High-Resolution Analysis of Plant Chromosomes -- Tyramide Signal Amplification: Fluorescence In Situ...”

Full text 

Table of Contents: “... with In Vivo Recombinase Mediated Gene Stacking -- Generation and Analysis of Transposon Ac/Ds-Induced Chromosomal Rearrangements in Rice Plants -- One-Step Generation of Chromosomal Rearrangements in Rice -- Genome Elimination by Tailswap CenH3: In Vivo Haploid...”

Full text 

Table of Contents: “... selectable genes -- VI Vectors for the integration of exogenous DNA into chromosomal DNA -- VII Conclusions -- VIII Acknowledgements -- IX References -- A comprehensive list of cloned eukaryotic genes -- Key....”

Full text 

Table of Contents: “... to Study Human Disease -- Physical Mapping of Plant Chromosomes by in Situ Hybridization -- Mutants and Variants of the Alcohol Dehydrogenase-1 Gene in Maize -- Developmentally Regulated Multigene Families in Dictyostelium Discoideum -- Computer Assisted Methods...”

Full text