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Tissue-Resident Macrophages [E-Book] : Methods and Protocols /
Table of Contents: ... Macrophages in Adult Zebrafish -- Genetic and Immunohistochemistry Tools to Visualize Rat Macrophages In Situ -- Phenotyping of Macrophages in Human Immune System Mice -- Fate-Mapping of Yolk Sac-Derived Macrophages -- Fate-Mapping of Hematopoietic Stem Cell...

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Cell Cycle Control [E-Book] : Methods and Protocols /
Table of Contents: ... Organisms -- Whole Mount Immunofluorescence Staining to Visualize Cell Cycle Progression in Mouse Oocyte Meiosis -- Imaging and Analysis of Drosophila Neural Stem Cell Asymmetric Division -- Cell Cycle Mapping Using Multiplexed Immunofluorescence -- Investigating...

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Trophoblasts [E-Book] : Methods and Protocols /
Table of Contents: ... to Study Human Placental Development and Function -- A Three-Dimensional Trophoblast Invasion Microfluidic Platform for Toxicological Screening -- Three-Dimensional In Vitro Human Placental Organoids from Mononuclear Villous Trophoblasts or Trophoblast Stem...

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Epithelial Cell Culture [E-Book] : Methods and Protocols /
Table of Contents: ... Liver Injury -- In Vitro Porcine (Explant) Colon Culture -- Bioencapsulation of Oocytes and Granulosa Cells -- Culturing and Differentiation of Patient-Derived Ectocervical Epithelial Stem Cells using Air-Liquid Interphase and Matrigel Scaffold -- Ovine...

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Familial Cardiomyopathies [E-Book] : Methods and Protocols /
Table of Contents: ... -- Genomic Engineering of Induced Pluripotent Stem Cell-Derived Cardiomyocytes -- The GENTIL Method for Isolation of Human Adult Cardiomyocytes from Cryopreserved Tissue for Proteomic Analyses -- Motility Assay to Probe the Calcium Sensitivity of Myosin...

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Embryo Models In Vitro [E-Book] : Methods and Protocols /
Table of Contents: ...Generation of Human Blastoids from Naïve Pluripotent Stem Cells -- Human Pre-Gastrulation Embryo Culture in 3D Condition -- Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System -- Generation of Human...

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Germ Cell Development [E-Book] : Methods and Protocols /
Table of Contents: ...Isolation and Purification of Viable PGCs from Mouse Embryos -- Purification and Transfection Methods of Chicken Primordial Germ Cells -- Isolation and In Vitro Propagation of Human Spermatogonial Stem Cells -- Purification by STA-PUT Technique of Male...

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Adipose-Derived Stem Cells [E-Book] : Methods and Protocols /
Table of Contents: ...Isolation of Human Adipose-Derived Stem Cells -- Isolation of Huma n Adipose-Derived Stem Cells from Lipoaspirates -- Isolation of Perivascular Mesenchymal Progenitor Cells from Human Adipose Tissue by Flow Cytometry -- Soft Tissue Reconstruction...

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Molecular Basis of Developmental and Stem Cell Regulation [E-Book] : Classical Models Revised /
Table of Contents: ...Part I. Somatic Cell Development from the Epiblast -- Chapter 1. The Epiblast and Pluripotent Stem Cell Lines -- Chapter 2. Different Types of Pluripotent Stem Cells Represent Different Developmental Stages -- Chapter 3. Gastrulation: Its Principles...

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Syncytia: Origin, Structure, and Functions [E-Book] /
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Cellular and Molecular Aspects of Ageing [E-Book] /
Table of Contents: ...: Ana Maria Cuervo -- 7. Deregulated nutrient-sensing: Pedro Manoel M.M.Vieira -- 8. Mitochondria/Oxidative Stress: Tiago Rodrigues -- 9. Stem cell exhaustion: Michael D Milsom -- 10. Cell-to-cell communication: Ana O'Loghlen -- 11. Senescence-associated...

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Cell Biology and Translational Medicine, Volume 21 [E-Book] : Stem Cell in Lineage, Secretome Regulation and Cancer /
Table of Contents: ...An Insight into Vital Genes Responsible for β-cell Formation -- Mesenchymal Stem Cells: A Promising Treatment for Thymic Involution -- Extracellular Vesicles in Domestic Animals: Cellular Communication in Health and Disease -- Secretome Analysis of Human...

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CCN Proteins [E-Book] : Methods and Protocols  /
Table of Contents: ... Effects by CCN2 Protein in Neuronal Precursor Cells Derived from Human Induced Pluripotent Stem Cells -- Gene Expression Analysis of CCNs Along with Odontoblastic Differentiation In Vivo -- Angiogenesis Assays for the Analysis of CCN Proteins -- Using...

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Golgi [E-Book] : Methods and Protocols /
Table of Contents: ... in Cell Polarity and Morphogenesis in Three-Dimensional Human Pluripotent Stem Cell Cysts -- Immunofluorescence Microscopy of the Mammalian Golgi Apparatus -- Studying the Organization of the Golgi by Super-Resolution Microscopy -- Super-Resolution Live Imaging...

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Poly(ADP-Ribose) Polymerase [E-Book] : Methods and Protocols /
Table of Contents: ... that Affect PARP1 Activity In Vivo -- Generating PARP Knockout D. Melanogaster with CRISPR/Cas9 System -- TaqMan Multiplex qPCR Method to Genotype PARG Knockout Mice -- Cell-Based Screening for New PARP Inhibitors Utilizing PARG Mutated Mouse Embryonic Stem Cells...

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Schmidtea Mediterranea [E-Book] : Methods and Protocols /
Table of Contents: ...-Content Fluorescence Microscopy Using RNA In Situ Hybridization and Immunocytochemistry -- An RNA/DNA-Based Flow Cytometry Approach for Isolating Slow-Cycling Stem Cells -- ACME Dissociation-Fixation, Flow Cytometry, and Cell Sorting of Freshwater Planarian...

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Retinal Ganglion Cells [E-Book] : Methods and Protocols /
Mead, Ben
2023
Table of Contents: ... Gun DiOlistic Labeling of Retinal Ganglion Cells -- Differentiation of Human Embryonic/Induced-Pluripotent Stem Cells to Retinal Ganglion Cells -- Laser-Induced Ocular Hypertension in a Mouse Model of Glaucoma -- Silicone Oil-Induced Ocular Hypertension...

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Cancer Cell Culture [E-Book] : Methods and Protocols  /
Table of Contents: ... Patients -- Serum-Free Production of Human Stem Cell-Derived Liver Spheres for Cancer Metastasis Research -- A Self-Assembly Method for Creating Vascularized Tumor Explants Using Biomaterials for 3D Culture -- A Step-by-Step Methodological Guide for Developing...

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Hepatic Stellate Cells [E-Book] : Methods and Protocols  /
Table of Contents: ...Isolation, Purification, and Culture of Primary Murine Hepatic Stellate Cells: An Update -- Differentiation of Hepatic Stellate Cells from Pluripotent Stem Cells -- Testing Cell Migration, Invasion, Proliferation, and Apoptosis in Hepatic Stellate Cells...

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Mechanobiology [E-Book] : Methods and Protocols  /
Table of Contents: ... Bilayers to Probe the Mechanosensitivity of Signaling Receptors -- Monitoring Mechano-Regulation of Gene Expression by RNA Sequencing -- Testing the Role of Focal Adhesion Kinase (FAK) in Topography-Mediated Stem Cell Differentiation by Inhibiting FAK...

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