Table of Contents: “...A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9 -- CRISPR/Cas9 Screening to Identify Conditionally Essential Genes in Human Cell Lines -- Target Identification of Small Molecules Using...”

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Table of Contents: “... starting with the embryo -- Computational methods to identify cell fate determinants, identity transcription factors and niche induced signaling pathways for stem cell research -- Differential proteomic analysis of complex mixtures by label free nLC MS/MS...”

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Table of Contents: “... pluripotent stem cells -- Microinjection of zygotes for CRISPR/Cas9 mediated insertion of transgenes into the murine Rosa26 safe harbour -- CRISPR-on for endogenous activation of SMARCA4 expression in bovine embryos -- CRISPR/Cas mutagenesis to generate novel...”

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Subject (ZB): “...Stem cells....”

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Table of Contents: “... Hydrogel Biodegradation -- Computational modeling and simulation to quantify the effects of obstructions on the performance of ventricular catheters used in hydrocephalus treatment -- Selection of Cancer Stem Cell--targeting Agents Using Bacteriophage Display...”

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Table of Contents: “... and Characterization of Human Pluripotent Stem Cell-Derived Cardiac Endothelial Cells for In Vitro Applications -- Finding and Verifying Enhancers for Endothelial-Expressed Genes -- Endothelial Cell RNA-Seq Data: Differential Expression and Functional Enrichment Analyses...”

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Table of Contents: “... of Human Natural Killer Cells under Defined Conditions by Seamless Transition from Maintenance Culture of Pluripotent Stem Cells -- Development of Humanized Mouse Models for Studying Human NK Cells in Health and Disease -- Antibody-Oligonucleotide Conjugation...”

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Table of Contents: “...Establishment of Mouse Epiblast Stem Cells -- L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Pluripotent State: The Early Primitive Ectoderm-Like Cell -- Generation of Epiblast-Like Cells -- Identification...”

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Table of Contents: “...Rapid and Highly Efficient Isolation and Purification of Human Induced Pluripotent Stem Cells -- Artificial Activation of Murine Oocytes using Strontium to Derive Haploid and Diploid Parthenotes -- Generation of Human iPSC from Small Volume Peripheral...”

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Table of Contents: “...CRISPR Library Screening in Cultured Cardiomyocytes -- Protein and mRNA Quantification in Small Samples of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 96-Well Microplates -- Self-Assembled Heterotypic Cardiac Spheroids from Human...”

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Table of Contents: “... approaches to obtain medulloblastoma stem cells from bulk cultures -- Drug Screening assays on Medulloblastoma Stem Cells using compound libraries -- 3D-on-2D: A physiologically-relevant and gel-free in vitro co-culture method to assay anti-metastatic agents...”

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Table of Contents: “..., and Approaches to a Cure -- Human Hematopoietic Stem Cell (HSC)-Engrafted NSG Mice for HIV Latency Research -- Inducing long-term HIV-1 latency in the TKO-BLT mouse model -- In situ multiplexing to identify, quantify, and phenotype the HIV-1/SIV reservoir within...”

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Table of Contents: “...Cerebellar Modeling using Human Induced Pluripotent Stem Cells -- From Cerebellar Genes to Behaviors in Zebrafish -- Silencing the Output of Cerebellar Neurons using Cell Type-Specific Genetic Deletion of Vesicular GABA and Glutamate Transporters...”

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Table of Contents: “... Materials for Medical and Engineering Applications -- Stem Cells and Proteomics in Biomaterials and Biomedical Applications -- Antimicrobial Bioceramics for Biomedical Applications -- Surface Functionalization of Titanium for the Control and Treatment...”

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Table of Contents: “... of Human Induced Pluripotent Stem Cells-Derived Liver Organoids -- Chromatin Immunoprecipitation Assay in Primary Mouse Hepatocytes and Mouse Liver -- Analysis of Liver Responses to Non-Alcoholic Steatohepatitis by mRNA-Sequencing -- Single-Cell RNA-Sequencing...”

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Table of Contents: “... -- Optogenetic Control of TGF-β Signaling -- Using Microfluidics and Live Cell Reporters to Dissect the Dynamics of TGF-β Signaling in Mouse Embryonic Stem Cells -- Energy Landscape Analysis of the Epithelial-Mesenchymal Transition Network -- Discrete Logic Modeling...”

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Table of Contents: “... using Botrylloides anceps -- In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis -- Isolation and Maintenance of In Vitro Cell Cultures from the Ctenophore Mnemiopsis leidyi -- Analysis of Spatial Gene Expression...”

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Table of Contents: “... -- Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons -- In vivo Live Imaging of Axonal Transport In Developing Zebrafish Axons -- Visualizing the Intracellular...”

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